Name: Anti-PES1 Antibody (CAB14506)
Brand: Cell Biology Antibodies 5
SKU: CAB14506
Availability: OutOfStock

Antibody Name:	Anti-PES1 Antibody
Antibody SKU:	CAB14506
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human PES1 (NP_055118.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human
Positive Samples:	MCF7, HepG2, Jurkat

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human PES1 (NP_055118.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MGGL EKKK YERG SATN YITR NKAR KKLQ LSLA DFRR LCIL KGIY PHEP KHKK KVNK GSTA ARTF YLIK DIRF LLHE PIVN KFRE YKVF VRKL RKAY GKSE WNTV ERLK DNKP NYKL DHII KERY PTFI DALR DLDD ALSM CFLF STFP RT
Gene ID:	23481
Uniprot:	O00541
Cellular Location:	Chromosome, Nucleus, nucleolus, nucleoplasm
Calculated MW:	67kDa/68kDa
Observed MW:	68kDa

Synonyms:

PES1, PES, NOP7

Background:

This gene encodes a nuclear protein that contains a breast cancer associated gene 1 (BRCA1) C-terminal interaction domain. The encoded protein interacts with BOP1 and WDR12 to form the PeBoW complex, which plays a critical role in cell proliferation via pre-rRNA processing and 60S ribosomal subunit maturation. Expression of this gene may play an important role in breast cancer proliferation and tumorigenicity. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Pseudogenes of this gene are located on the long arm of chromosome 4 and the short arm of chromosome 9.

UniProt Protein Function:	PES1: Component of the PeBoW complex, which is required for maturation of 28S and 5.8S ribosomal RNAs and formation of the 60S ribosome. Belongs to the pescadillo family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Translation; RNA-binding; Nucleolus Chromosomal Location of Human Ortholog: 22q12.1 Cellular Component: cytoplasm; membrane; nucleolus; nucleoplasm; nucleus Molecular Function:protein binding; RNA binding Biological Process: cell proliferation; maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); regulation of cell cycle; ribosomal large subunit biogenesis and assembly; rRNA processing
NCBI Summary:	This gene encodes a nuclear protein that contains a breast cancer associated gene 1 (BRCA1) C-terminal interaction domain. The encoded protein interacts with BOP1 and WDR12 to form the PeBoW complex, which plays a critical role in cell proliferation via pre-rRNA processing and 60S ribosomal subunit maturation. Expression of this gene may play an important role in breast cancer proliferation and tumorigenicity. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Pseudogenes of this gene are located on the long arm of chromosome 4 and the short arm of chromosome 9. [provided by RefSeq, Aug 2011]
UniProt Code:	O00541
NCBI GenInfo Identifier:	21542165
NCBI Gene ID:	23481
NCBI Accession:	O00541.1
UniProt Secondary Accession:	O00541,Q6IC29,
UniProt Related Accession:	O00541
Molecular Weight:	67,456 Da
NCBI Full Name:	Pescadillo homolog
NCBI Synonym Full Names:	pescadillo ribosomal biogenesis factor 1
NCBI Official Symbol:	PES1
NCBI Official Synonym Symbols:	PES
NCBI Protein Information:	pescadillo homolog
UniProt Protein Name:	Pescadillo homolog
UniProt Gene Name:	PES1
UniProt Entry Name:	PESC_HUMAN

Western blot analysis of extracts of various cell lines, using PES1 antibody (CAB14506) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s.

Antibodies

PES1 Antibody (PACO11171)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)