Anti-Perilipin-2 Antibody (CAB20843)
- SKU:
- CAB20843
- Product Type:
- Antibody
- Antibody Type:
- Polyclonal Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
Description
| Antibody Name: | Anti-Perilipin-2 Antibody (CAB20843) |
| Antibody SKU: | CAB20843 |
| Antibody Size: | 50µL, 100µL |
| Application: | Western blotting, Immunohistochemistry, Immunofluorescence |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 88-437 of human Perilipin-2 (NP_001113.2). |
| Application: | Western blotting, Immunohistochemistry, Immunofluorescence |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | HepG2, Jurkat |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 88-437 of human Perilipin-2 (NP_001113.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | DRIE ERLP ILNQ PSTQ IVAN AKGA VTGA KDAV TTTV TGAK DSVA STIT GVMD KTKG AVTG SVEK TKSV VSGS INTV LGSR MMQL VSSG VENA LTKS ELLV EQYL PLTE EELE KEAK KVEG FDLV QKPS YYVR LGSL STKL HSRA YQQA LSRV KEAK QKSQ QTIS QLHS TVHL IEFA RKNV YSAN QKIQ DAQD KLYL SWVE WKRS IGYD DTDE SHCA EHIE SRTL AIAR NLTQ QLQT TCHT LLSN IQGV PQNI QDQA KHMG VMAG DIYS VFRN AASF KEVS DSLL TSSK GQLQ KMKE SLDD VMDY LVNN TPLN WLVG PFYP QLTE SQNA QDQG AEMD KSSQ ETQR SEHK TH |
| Cellular Location: | Membrane, Peripheral membrane protein |
| Calculated MW: | 48kDa |
| Observed MW: | 52KDa |
| Synonyms: | PLIN2, ADFP, ADRP |
| Background: | The protein encoded by this gene belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases. Alternatively spliced transcript variants have been found for this gene. |
 | Immunofluorescence analysis of HepG2 cells using Perilipin-2 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of Mouse brown adipose using Perilipin-2 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunohistochemistry of paraffin-embedded human colon carcinoma using Perilipin-2 antibody at dilution of 1:1600 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. |
 | Immunohistochemistry of paraffin-embedded human liver using Perilipin-2 antibody at dilution of 1:1600 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. |
 | Western blot analysis of extracts of various cell lines, using Perilipin-2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s. |
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