Antibody Name:	Anti-PER2 Antibody
Antibody SKU:	CAB13168
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human PER2 (NP_073728.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human PER2 (NP_073728.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MNGY AEFP PSPS NPTK EPVE PQPS QVPL QEDV DMSS GSSG HETN ENCS TGRD SQGS DCDD SGKE LGML VEPP DARQ SPDT FSLM MAKS EHNP STSG CSSD QSSK VDTH KELI KTLK ELKV HLPA DKKA KGKA STLA TLKY ALRS VKQV KA
Gene ID:	8864
Uniprot:	O15055
Cellular Location:	Cytoplasm, Nucleus, nucleolus, perinuclear region
Calculated MW:	45kDa/136kDa
Observed MW:	136KDa

Synonyms:

PER2, FASPS, FASPS1

Background:

This gene is a member of the Period family of genes and is expressed in a circadian pattern in the suprachiasmatic nucleus, the primary circadian pacemaker in the mammalian brain. Genes in this family encode components of the circadian rhythms of locomotor activity, metabolism, and behavior. This gene is upregulated by CLOCK/ARNTL heterodimers but then represses this upregulation in a feedback loop using PER/CRY heterodimers to interact with CLOCK/ARNTL. Polymorphisms in this gene may increase the risk of getting certain cancers and have been linked to sleep disorders.

UniProt Protein Function:	PER2: a transcription factor belonging to the basic helix-loop-helix (bHLH) family. A member of the Period family of genes and is expressed in a circadian pattern in the suprachiasmatic nucleus, the primary circadian pacemaker in the mammalian brain. Genes in this family encode components of the circadian rhythms of locomotor activity, metabolism, and behavior. Circadian expression in the suprachiasmatic nucleus continues in constant darkness, and a shift in the light/dark cycle evokes a proportional shift of gene expression in the suprachiasmatic nucleus. Behaves as a negative element in circadian transcriptional loop. Does not appear to bind DNA, suggesting indirect transcriptional inhibition. Expression oscillates with a 24 hr rhythm in the suprachiasmatic nucleus and the whole eyes. Oscillations are maintained under constant darkness and are responsive to changes of the light/dark cycles. There is a 4 hour time delay between per1 and per2 oscillations. The expression rhythms appear to originate from retina.
UniProt Protein Details:	Protein type:Nucleolus; Nuclear receptor co-regulator; Transcription factor Chromosomal Location of Human Ortholog: 2q37.3 Cellular Component: cytoplasm; nucleolus; nucleoplasm; nucleus; perinuclear region of cytoplasm Molecular Function:protein binding; transcription coactivator activity; ubiquitin binding Biological Process: circadian regulation of gene expression; circadian rhythm; fatty acid metabolic process; gluconeogenesis; glycogen biosynthetic process; lactate biosynthetic process; negative regulation of circadian rhythm; negative regulation of protein ubiquitination; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; regulation of cell cycle; regulation of circadian rhythm; regulation of glutamate uptake during transmission of nerve impulse; regulation of insulin secretion; regulation of neurogenesis; regulation of vasoconstriction; transcription, DNA-dependent; white fat cell differentiation Disease: Advanced Sleep Phase Syndrome, Familial, 1
NCBI Summary:	This gene is a member of the Period family of genes and is expressed in a circadian pattern in the suprachiasmatic nucleus, the primary circadian pacemaker in the mammalian brain. Genes in this family encode components of the circadian rhythms of locomotor activity, metabolism, and behavior. This gene is upregulated by CLOCK/ARNTL heterodimers but then represses this upregulation in a feedback loop using PER/CRY heterodimers to interact with CLOCK/ARNTL. Polymorphisms in this gene may increase the risk of getting certain cancers and have been linked to sleep disorders. [provided by RefSeq, Jan 2014]
UniProt Code:	O15055
NCBI GenInfo Identifier:	14917029
NCBI Gene ID:	8864
NCBI Accession:	O15055.2
UniProt Secondary Accession:	O15055,Q4ZG49, Q6DT41, Q9UQ45, A2I2P7,
UniProt Related Accession:	O15055
Molecular Weight:
NCBI Full Name:	Period circadian protein homolog 2
NCBI Synonym Full Names:	period circadian clock 2
NCBI Official Symbol:	PER2Â Â
NCBI Official Synonym Symbols:	FASPS; FASPS1Â Â
NCBI Protein Information:	period circadian protein homolog 2
UniProt Protein Name:	Period circadian protein homolog 2
UniProt Synonym Protein Names:	Circadian clock protein PERIOD 2
Protein Family:	Period circadian protein
UniProt Gene Name:	PER2Â Â
UniProt Entry Name:	PER2_HUMAN

	Western blot analysis of extracts of 293T cells, using PER2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
	Western blot analysis of extracts of Mouse liver, using PER2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat spleen using PER2 Rabbit pAb at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
	Immunohistochemistry of paraffin-embedded human thyroid cancer using PER2 Rabbit pAb at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
	Immunohistochemistry of paraffin-embedded mouse testis using PER2 Rabbit pAb at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
	Chromatin immunoprecipitation analysis of extracts of MCF7 cells, using PER2 antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.