Cell Biology Antibodies 12

Anti-PER1 Antibody (CAB8449)

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SKU:
CAB8449
Product Type:
Antibody
Applications:
WB
Applications:
IHC
Applications:
IF
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-PER1 Antibody
Antibody SKU:CAB8449
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human PER1 (NP_002607.2).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:Jurkat, B cells, A-549, Mouse brain, Mouse testis, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human PER1 (NP_002607.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MSGP LEGA DGGG DPRP GESF CPGG VPSP GPPQ HRPC PGPS LADD TDAN SNGS SGNE SNGH ESRG ASQR SSHS SSSG NGKD SALL ETTE SSKS TNSQ SPSP PSSS IAYS LLSA SSEQ DNPS TSGC SSEQ SARA RTQK ELMT ALRE LKLR LPPE RRGK GRSG TLAT LQYA LACV KQVQ ANQE YYQQ WSLE EGEP CSMD MSTY
Gene ID:5187
Uniprot:O15534
Cellular Location:Cytoplasm, Nucleus
Calculated MW:91kDa/136kDa
Observed MW:160kDa
Synonyms:PER1, PER, RIGUI, hPER
Background:This gene is a member of the Period family of genes and is expressed in a circadian pattern in the suprachiasmatic nucleus, the primary circadian pacemaker in the mammalian brain. Genes in this family encode components of the circadian rhythms of locomotor activity, metabolism, and behavior. This gene is upregulated by CLOCK/ARNTL heterodimers but then represses this upregulation in a feedback loop using PER/CRY heterodimers to interact with CLOCK/ARNTL. Polymorphisms in this gene may increase the risk of getting certain cancers. Alternative splicing has been observed in this gene; however, these variants have not been fully described.
UniProt Protein Function:PER1: Component of the circadian clock mechanism which is essential for generating circadian rhythms. Negative element in the circadian transcriptional loop. Influences clock function by interacting with other circadian regulatory proteins and transporting them to the nucleus. Negatively regulates CLOCK|NPAS2-BMAL1|BMAL2-induced transactivation. Can bind heme. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Transcription factor

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: cytoplasm

Molecular Function:chromatin DNA binding; kinase binding; ubiquitin protein ligase binding

Biological Process: circadian regulation of gene expression; circadian rhythm; entrainment of circadian clock; entrainment of circadian clock by photoperiod; negative regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of JNK cascade; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; regulation of circadian rhythm; regulation of hair cycle; regulation of sodium ion transport

NCBI Summary:This gene is a member of the Period family of genes and is expressed in a circadian pattern in the suprachiasmatic nucleus, the primary circadian pacemaker in the mammalian brain. Genes in this family encode components of the circadian rhythms of locomotor activity, metabolism, and behavior. This gene is upregulated by CLOCK/ARNTL heterodimers but then represses this upregulation in a feedback loop using PER/CRY heterodimers to interact with CLOCK/ARNTL. Polymorphisms in this gene may increase the risk of getting certain cancers. Alternative splicing has been observed in this gene; however, these variants have not been fully described. [provided by RefSeq, Jan 2014]
UniProt Code:O15534
NCBI GenInfo Identifier:218511752
NCBI Gene ID:5187
NCBI Accession:O15534.2
UniProt Secondary Accession:O15534,B2RPA8, B4DI49, D3DTR3,
UniProt Related Accession:O15534
Molecular Weight:
NCBI Full Name:Period circadian protein homolog 1
NCBI Synonym Full Names:period circadian clock 1
NCBI Official Symbol:PER1  
NCBI Official Synonym Symbols:PER; hPER; RIGUI  
NCBI Protein Information:period circadian protein homolog 1
UniProt Protein Name:Period circadian protein homolog 1
UniProt Synonym Protein Names:Circadian clock protein PERIOD 1; Circadian pacemaker protein Rigui
Protein Family:Protein
UniProt Gene Name:PER1  
UniProt Entry Name:PER1_HUMAN
Anti-PER1 Polyclonal Antibody (CAB8449)Western blot analysis of extracts of various cell lines, using PER1 antibody (CAB8449) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
Anti-PER1 Polyclonal Antibody (CAB8449)Immunohistochemistry of paraffin-embedded human stomach using PER1 antibody (CAB8449) at dilution of 1:100 (40x lens).
Anti-PER1 Polyclonal Antibody (CAB8449)Immunohistochemistry of paraffin-embedded rat kidney using PER1 antibody (CAB8449) at dilution of 1:100 (40x lens).
Anti-PER1 Polyclonal Antibody (CAB8449)Immunohistochemistry of paraffin-embedded mouse stomach using PER1 antibody (CAB8449) at dilution of 1:100 (40x lens).
Anti-PER1 Polyclonal Antibody (CAB8449)Immunofluorescence analysis of U2OS cells using PER1 antibody (CAB8449) at dilution of 1:100. Blue: DAPI for nuclear staining.
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