Cell Biology Antibodies 8

Anti-Pepsinogen II Antibody (CAB2788)

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SKU:
CAB2788
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-Pepsinogen II Antibody
Antibody SKU:CAB2788
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 100-200 of human Pepsinogen II (NP_001159896.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:100 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:Mouse liver, Mouse heart, Rat liver
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 100-200 of human Pepsinogen II (NP_001159896.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:PSVY CQSQ ACTS HSRF NPSE SSTY STNG QTFS LQYG SGSL TGFF GYDT LTVQ SIQV PNQE FGLS ENEP GTNF VYAQ FDGI MGLA YPAL SVDE ATTA MQGM V
Gene ID:5225
Uniprot:P20142
Cellular Location:Secreted
Calculated MW:34kDa/42kDa
Observed MW:45-50kDa
Synonyms:PGC, PEPC, PGII, gastricsin
Background:This gene encodes an aspartic proteinase that belongs to the peptidase family A1. The encoded protein is a digestive enzyme that is produced in the stomach and constitutes a major component of the gastric mucosa. This protein is also secreted into the serum. This protein is synthesized as an inactive zymogen that includes a highly basic prosegment. This enzyme is converted into its active mature form at low pH by sequential cleavage of the prosegment that is carried out by the enzyme itself. Polymorphisms in this gene are associated with susceptibility to gastric cancers. Serum levels of this enzyme are used as a biomarker for certain gastric diseases including Helicobacter pylori related gastritis. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 1.
UniProt Protein Function:PGC: Hydrolyzes a variety of proteins. Belongs to the peptidase A1 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Secreted, signal peptide; Protease; Secreted; EC 3.4.23.3

Chromosomal Location of Human Ortholog: 6p21.1

Cellular Component: extracellular space

Molecular Function:aspartic-type endopeptidase activity

Biological Process: digestion; proteolysis

NCBI Summary:This gene encodes an aspartic proteinase that belongs to the peptidase family A1. The encoded protein is a digestive enzyme that is produced in the stomach and constitutes a major component of the gastric mucosa. This protein is also secreted into the serum. This protein is synthesized as an inactive zymogen that includes a highly basic prosegment. This enzyme is converted into its active mature form at low pH by sequential cleavage of the prosegment that is carried out by the enzyme itself. Polymorphisms in this gene are associated with susceptibility to gastric cancers. Serum levels of this enzyme are used as a biomarker for certain gastric diseases including Helicobacter pylori related gastritis. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 1. [provided by RefSeq, Oct 2009]
UniProt Code:P20142
NCBI GenInfo Identifier:129796
NCBI Gene ID:5225
NCBI Accession:P20142.1
UniProt Secondary Accession:P20142,Q5T3D7, Q5T3D8, B4DVZ3,
UniProt Related Accession:P20142
Molecular Weight:40,600
NCBI Full Name:Gastricsin
NCBI Synonym Full Names:progastricsin (pepsinogen C)
NCBI Official Symbol:PGC  
NCBI Official Synonym Symbols:PEPC; PGII  
NCBI Protein Information:gastricsin; pepsin C; pepsinogen C; preprogastricsin; pepsinogen group II
UniProt Protein Name:Gastricsin
UniProt Synonym Protein Names:Pepsinogen C
Protein Family:Gastricsin
UniProt Gene Name:PGC  
UniProt Entry Name:PEPC_HUMAN
Anti-Pepsinogen II Antibody (CAB2788)Western blot analysis of extracts of various cell lines, using Pepsinogen II antibody (CAB2788) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
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