Cell Biology Antibodies 9
Anti-PEPD Antibody (CAB5416)
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- SKU:
- CAB5416
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-PEPD Antibody |
| Antibody SKU: | CAB5416 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 288-424 of human PEPD (NP_000276.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 293T, HT-29, BT-474, Mouse kidney, Mouse liver, Rat kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 288-424 of human PEPD (NP_000276.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | ITCS FPAN GKFT ADQK AVYE AVLR SSRA VMGA MKPG VWWP DMHR LADR IHLE ELAH MGIL SGSV DAMV QAHL GAVF MPHG LGHF LGID VHDV GGYP EGVE RIDE PGLR SLRT ARHL QPGM VLTV EPGI YFID HLLD E |
| Gene ID: | 5184 |
| Uniprot: | P12955 |
| Cellular Location: | |
| Calculated MW: | 47kDa/49kDa/54kDa |
| Observed MW: | 54kDa |
| Synonyms: | PEPD, PROLIDASE |
| Background: | This gene encodes a member of the peptidase family. The protein forms a homodimer that hydrolyzes dipeptides or tripeptides with C-terminal proline or hydroxyproline residues. The enzyme serves an important role in the recycling of proline, and may be rate limiting for the production of collagen. Mutations in this gene result in prolidase deficiency, which is characterized by the excretion of large amount of di- and tri-peptides containing proline. Multiple transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | peptidase D: Splits dipeptides with a prolyl or hydroxyprolyl residue in the C-terminal position. Plays an important role in collagen metabolism because the high level of iminoacids in collagen. Defects in PEPD are a cause of prolidase deficiency (PD). Prolidase deficiency is an autosomal recessive disorder associated with iminodipeptiduria. The clinical phenotype includes skin ulcers, mental retardation, recurrent infections, and a characteristic facies. These features, however are incompletely penetrant and highly variable in both age of onset and severity. There is a tight linkage between the polymorphisms of prolidase and the myotonic dystrophy trait. Belongs to the peptidase M24B family. Eukaryotic-type prolidase subfamily. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 3.4.13.9; Protease Chromosomal Location of Human Ortholog: 19q13.11 Molecular Function:dipeptidase activity; manganese ion binding; aminopeptidase activity; metallocarboxypeptidase activity Biological Process: amino acid metabolic process; collagen catabolic process; proteolysis Disease: Prolidase Deficiency |
| NCBI Summary: | This gene encodes a member of the peptidase family. The protein forms a homodimer that hydrolyzes dipeptides or tripeptides with C-terminal proline or hydroxyproline residues. The enzyme serves an important role in the recycling of proline, and may be rate limiting for the production of collagen. Mutations in this gene result in prolidase deficiency, which is characterized by the excretion of large amount of di- and tri-peptides containing proline. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2009] |
| UniProt Code: | P12955 |
| NCBI GenInfo Identifier: | 50403718 |
| NCBI Gene ID: | 5184 |
| NCBI Accession: | P12955.3 |
| UniProt Secondary Accession: | P12955,Q8TBN9, Q9BT75, A8K3Z1, A8K416, A8K696, A8MX47 B4DDB7, B4DGJ1, E9PCE8, |
| UniProt Related Accession: | P12955 |
| Molecular Weight: | 493 |
| NCBI Full Name: | Xaa-Pro dipeptidase |
| NCBI Synonym Full Names: | peptidase D |
| NCBI Official Symbol: | PEPD |
| NCBI Official Synonym Symbols: | PROLIDASE |
| NCBI Protein Information: | xaa-Pro dipeptidase; imidodipeptidase; X-Pro dipeptidase; proline dipeptidase; aminoacyl-L-proline hydrolase |
| UniProt Protein Name: | Xaa-Pro dipeptidase |
| UniProt Synonym Protein Names: | Imidodipeptidase; Peptidase D; Proline dipeptidase; Prolidase |
| Protein Family: | Dipeptidase |
| UniProt Gene Name: | PEPD |
| UniProt Entry Name: | PEPD_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PEPD antibody (CAB5416) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s. |
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