Signal Transduction Antibodies 3

Anti-PELI3 Antibody (CAB9999)

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SKU:
CAB9999
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Signal Transduction

Description

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Antibody Name:Anti-PELI3 Antibody
Antibody SKU:CAB9999
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human PELI3 (NP_659502.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:HepG2, A-549, SGC-7901, HeLa
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human PELI3 (NP_659502.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MVLE GNPE VGSP RTSD LQHR GNKG SCVL SSPG EDAQ PGEE PIKY GELI VLGC CEEG GEET EAQR GEVT GPRA HSCY NGCL ASGD KGRR RSRL ALSR RSHA NGVK PDVM HHIS TPLV SKAL SNRG QHSI SYTL SRSH SVIV EYTH DSDT DMFQ IGRS TENM IDFV VTDT SPGG GAAE GPSA QSTI SRYA CRIL CDRR PPYT ARIY AAGF DASS NIFL GERA AKWR TPDG LMDG LTTN GVLV MHPA GGFS EDSA PGVW REIS VCGN VYTL RDSR SAQQ RGKL
Gene ID:246330
Uniprot:Q8N2H9
Cellular Location:
Calculated MW:35kDa/43kDa/48kDa/50kDa
Observed MW:50kDa
Synonyms:PELI3
Background:The protein encoded by this gene is a scaffold protein and an intermediate signaling protein in the innate immune response pathway. The encoded protein helps transmit the immune response signal from Toll-like receptors to IRAK1/TRAF6 complexes. Several transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:PELI3: E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. Involved in the TLR and IL-1 signaling pathways via interaction with the complex containing IRAK kinases and TRAF6. Mediates 'Lys-63'-linked polyubiquitination of IRAK1. Can activate AP1/JUN and ELK1. Not required for NF-kappa-B activation. Belongs to the pellino family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Adaptor/scaffold; EC 6.3.2.-; EC 6.3.2.19; Ligase; Ubiquitin conjugating system; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 11q13.2

Cellular Component: cytosol

Molecular Function:protein binding; protein serine/threonine kinase activity

Biological Process: immune response; Toll signaling pathway

NCBI Summary:The protein encoded by this gene is a scaffold protein and an intermediate signaling protein in the innate immune response pathway. The encoded protein helps transmit the immune response signal from Toll-like receptors to IRAK1/TRAF6 complexes. Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011]
UniProt Code:Q8N2H9
NCBI GenInfo Identifier:37999712
NCBI Gene ID:246330
NCBI Accession:Q8N2H9.2
UniProt Secondary Accession:Q8N2H9,Q8N3E1, Q8N9Q6, Q8TAW7, Q8TED5,
UniProt Related Accession:Q8N2H9
Molecular Weight:51kDa
NCBI Full Name:E3 ubiquitin-protein ligase pellino homolog 3
NCBI Synonym Full Names:pellino E3 ubiquitin protein ligase family member 3
NCBI Official Symbol:PELI3  
NCBI Protein Information:E3 ubiquitin-protein ligase pellino homolog 3
UniProt Protein Name:E3 ubiquitin-protein ligase pellino homolog 3
UniProt Synonym Protein Names:RING-type E3 ubiquitin transferase pellino homolog 3Curated
Protein Family:E3 ubiquitin-protein ligase
UniProt Gene Name:PELI3  
Anti-PELI3 Polyclonal Antibody (CAB9999)Western blot analysis of extracts of various cell lines, using PELI3 Antibody (CAB9999) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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