Antibody Name:	Anti-PEG3 Antibody
Antibody SKU:	CAB12671
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PEG3 (NP_006201.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HepG2

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PEG3 (NP_006201.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MLPP KHLS ATKP KKSW APNL YELD SDLT KEPD VIIG EGPT DSEF FHQR FRNL IYVE FVGP RKTL IKLR NLCL DWLQ PETR TKEE IIEL LVLE QYLT IIPE KLKP WVRA KKPE NCEK LVTL LENY KEMY QPED DNNS DVTS DDDM TRNR RESS PPHS VHSF SDRD WDRR GRSR DMEP RDRW SHTR NPRS RMPP RDLS LPVV AKTS FEMD REDD RDSR AYES RSQD AESY QNVV DLAE DRKP HNTI QDNM ENYR KLLS LVQL AEDD GHSH MTQG HSSR SKRS AYPS TSRG LKTM PEAK KSTH
Gene ID:	5178
Uniprot:	Q9GZU2
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	60kDa/165kDa/166kDa/180kDa
Observed MW:	181KDa

Synonyms:

PEG3, PW1, ZKSCAN22, ZNF904, ZSCAN24

Background:

In human, ZIM2 and PEG3 are treated as two distinct genes though they share multiple 5' exons and a common promoter and both genes are paternally expressed (PMID:15203203). Alternative splicing events connect their shared 5' exons either with the remaining 4 exons unique to ZIM2, or with the remaining 2 exons unique to PEG3. In contrast, in other mammals ZIM2 does not undergo imprinting and, in mouse, cow, and likely other mammals as well, the ZIM2 and PEG3 genes do not share exons. Human PEG3 protein belongs to the Kruppel C2H2-type zinc finger protein family. PEG3 may play a role in cell proliferation and p53-mediated apoptosis. PEG3 has also shown tumor suppressor activity and tumorigenesis in glioma and ovarian cells. Alternative splicing of this PEG3 gene results in multiple transcript variants encoding distinct isoforms.

UniProt Protein Function:	Induces apoptosis in cooperation with SIAH1A. Acts as a mediator between p53/TP53 and BAX in a neuronal death pathway that is activated by DNA damage. Acts synergistically with TRAF2 and inhibits TNF induced apoptosis through activation of NF-kappa-B. Possesses a tumor suppressing activity in glioma cells.
NCBI Summary:	In human, ZIM2 and PEG3 are treated as two distinct genes though they share multiple 5' exons and a common promoter and both genes are paternally expressed (PMID:15203203). Alternative splicing events connect their shared 5' exons either with the remaining 4 exons unique to ZIM2, or with the remaining 2 exons unique to PEG3. In contrast, in other mammals ZIM2 does not undergo imprinting and, in mouse, cow, and likely other mammals as well, the ZIM2 and PEG3 genes do not share exons. Human PEG3 protein belongs to the Kruppel C2H2-type zinc finger protein family. PEG3 may play a role in cell proliferation and p53-mediated apoptosis. PEG3 has also shown tumor suppressor activity and tumorigenesis in glioma and ovarian cells. Alternative splicing of this PEG3 gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Sep 2009]
UniProt Code:	Q9GZU2
NCBI GenInfo Identifier:	226053126
NCBI Gene ID:	5178
NCBI Accession:	NP_001139656.1
UniProt Secondary Accession:	Q9GZU2,P78418, Q5H9P9, Q7Z7H7, Q8TF75, Q9GZY2, A7E2B8 B4DIM4, C9JP50,
UniProt Related Accession:	Q9GZU2
Molecular Weight:
NCBI Full Name:	paternally-expressed gene 3 protein isoform 1
NCBI Synonym Full Names:	paternally expressed 3
NCBI Official Symbol:	PEG3
NCBI Official Synonym Symbols:	PW1; ZNF904; ZSCAN24; ZKSCAN22
NCBI Protein Information:	paternally-expressed gene 3 protein
UniProt Protein Name:	Paternally-expressed gene 3 protein
UniProt Synonym Protein Names:	Zinc finger and SCAN domain-containing protein 24
Protein Family:	Paternally-expressed gene 3 protein
UniProt Gene Name:	PEG3

	Western blot analysis of extracts of HepG2 cells, using PEG3 antibody (CAB12671) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded mouse brain using PEG3 Rabbit pAb (CAB12671) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat brain using PEG3 Rabbit pAb (CAB12671) at dilution of 1:100 (40x lens).