Cell Biology Antibodies 7

Anti-PEDF Antibody (CAB1673)

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SKU:
CAB1673
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-PEDF Antibody
Antibody SKU:CAB1673
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human PEDF (NP_002606.3).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:HepG2, Mouse liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human PEDF (NP_002606.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MQAL VLLL CIGA LLGH SSCQ NPAS PPEE GSPD PDST GALV EEED PFFK VPVN KLAA AVSN FGYD LYRV RSST SPTT NVLL SPLS VATA LSAL SLGA EQRT ESII HRAL YYDL ISSP DIHG TYKE LLDT VTAP QKNL KSAS RIVF EKKL RIKS SFVA PLEK SYGT RPRV LTGN PRLD LQEI NNWV QAQM KGKL ARST KEIP
Gene ID:5176
Uniprot:P36955
Cellular Location:Melanosome, Secreted
Calculated MW:46kDa
Observed MW:46KDa
Synonyms:SERPINF1, EPC-1, OI12, OI6, PEDF, PIG35
Background:The protein encoded by this gene is a member of the serpin family, although it does not display the serine protease inhibitory activity shown by many of the other serpin family members. The encoded protein is secreted and strongly inhibits angiogenesis. In addition, this protein is a neurotrophic factor involved in neuronal differentiation in retinoblastoma cells.
UniProt Protein Function:PEDF: a secreted neurotrophic protein that induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity. The N-terminal (AA 44-121) exhibits neurite outgrowth-inducing activity. The C-terminal exposed loop (AA 382-418) is essential for serpin activity. Extracellular phosphorylation enhances antiangiogenic activity.
UniProt Protein Details:

Protein type:Secreted; Secreted, signal peptide; Inhibitor

Chromosomal Location of Human Ortholog: 17p13.3

Cellular Component: axon hillock; extracellular matrix; extracellular space; perinuclear region of cytoplasm; extracellular region; melanosome; basement membrane

Molecular Function:serine-type endopeptidase inhibitor activity

Biological Process: cell proliferation; positive regulation of neurogenesis; negative regulation of angiogenesis; ovulation cycle; retina development in camera-type eye; short-term memory; response to arsenic; multicellular organismal development; negative regulation of inflammatory response; response to acidity; kidney development; aging

Disease: Osteogenesis Imperfecta, Type Vi

NCBI Summary:The protein encoded by this gene is a member of the serpin family, although it does not display the serine protease inhibitory activity shown by many of the other serpin family members. The encoded protein is secreted and strongly inhibits angiogenesis. In addition, this protein is a neurotrophic factor involved in neuronal differentiation in retinoblastoma cells.[provided by RefSeq, Mar 2011]
UniProt Code:P36955
NCBI GenInfo Identifier:313104314
NCBI Gene ID:5176
NCBI Accession:P36955.4
UniProt Secondary Accession:P36955,Q13236, Q2TU83, Q96CT1, Q96R01, Q9BWA4, F1T092
UniProt Related Accession:P36955
Molecular Weight:46,312 Da
NCBI Full Name:Pigment epithelium-derived factor
NCBI Synonym Full Names:serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1
NCBI Official Symbol:SERPINF1  
NCBI Official Synonym Symbols:OI6; OI12; PEDF; EPC-1; PIG35  
NCBI Protein Information:pigment epithelium-derived factor; serpin F1; cell proliferation-inducing gene 35 protein; serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1
UniProt Protein Name:Pigment epithelium-derived factor
UniProt Synonym Protein Names:Cell proliferation-inducing gene 35 protein; EPC-1; Serpin F1
UniProt Gene Name:SERPINF1  
UniProt Entry Name:PEDF_HUMAN
Anti-PEDF Antibody (CAB1673)Western blot analysis of extracts of various cell lines, using PEDF antibody (CAB1673) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
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