Cell Biology Antibodies 1

Anti-PEBP1 Antibody (CAB0578)

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SKU:
CAB0578
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-PEBP1 Antibody
Antibody SKU:CAB0578
Antibody Size:20uL, 50uL, 100uL
Application:WB IF IP
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-187 of human PEBP1 (NP_002558.1).
Application:WB IF IP
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200 IP 1:20 - 1:50
Reactivity:Human, Mouse, Rat
Positive Samples:BT-474, 293T, 22Rv1, A-549, PC-3, MCF7, Mouse liver, Mouse brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-187 of human PEBP1 (NP_002558.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MPVD LSKW SGPL SLQE VDEQ PQHP LHVT YAGA AVDE LGKV LTPT QVKN RPTS ISWD GLDS GKLY TLVL TDPD APSR KDPK YREW HHFL VVNM KGND ISSG TVLS DYVG SGPP KGTG LHRY VWLV YEQD RPLK CDEP ILSN RSGD HRGK FKVA SFRK KYEL RAPV AGTC YQAE WDDY VPKL YEQL SGK
Gene ID:5037
Uniprot:P30086
Cellular Location:Cytoplasm
Calculated MW:21kDa
Observed MW:22kDa
Synonyms:PEBP1, HCNP, HCNPpp, HEL-210, HEL-S-34, HEL-S-96, PBP, PEBP, PEBP-1, RKIP
Background:This gene encodes a member of the phosphatidylethanolamine-binding family of proteins and has been shown to modulate multiple signaling pathways, including the MAP kinase (MAPK), NF-kappa B, and glycogen synthase kinase-3 (GSK-3) signaling pathways. The encoded protein can be further processed to form a smaller cleavage product, hippocampal cholinergic neurostimulating peptide (HCNP), which may be involved in neural development. This gene has been implicated in numerous human cancers and may act as a metastasis suppressor gene. Multiple pseudogenes of this gene have been identified in the genome.
UniProt Protein Function:RKIP: a metastasis suppressor that regulates extracellular matrix remodeling and tumor survival. Binds to and inhibits Raf-1. Phosphorylation by classic and atypical but not novel PKC isoforms dissociates this complex, relieving inhibition of the Raf/MAP kinase signaling cascade. Binds ATP, opioids and phosphatidylethanolamine. Has lower affinity for phosphatidylinositol and phosphatidylcholine. Serine protease inhibitor which inhibits thrombin, neuropsin and chymotrypsin but not trypsin, tissue type plasminogen activator and elastase Inhibits the kinase activity of RAF1 by inhibiting its activation and by dissociating the RAF1/MEK complex and acting as a competitive inhibitor of MEK phosphorylation. Cleaved into hippocampal cholinergic neurostimulating peptide (HCMP) that appears to be involved in the function of the presynaptic cholinergic neurons of the central nervous system. HCNP increases the production of choline acetyltransferase but not acetylcholinesterase. Expressed in brain. Increased expression in aged senescence-accelerated mice.
UniProt Protein Details:

Protein type:Lipid-binding; Protein kinase, regulatory subunit

Chromosomal Location of Human Ortholog: 12q24.23

Cellular Component: cytosol; nucleus

Molecular Function:enzyme binding; phosphatidylethanolamine binding; protein binding; protein kinase binding

Biological Process: MAPKKK cascade

NCBI Summary:This gene encodes a member of the phosphatidylethanolamine-binding family of proteins and has been shown to modulate multiple signaling pathways, including the MAP kinase (MAPK), NF-kappa B, and glycogen synthase kinase-3 (GSK-3) signaling pathways. The encoded protein can be further processed to form a smaller cleavage product, hippocampal cholinergic neurostimulating peptide (HCNP), which may be involved in neural development. This gene has been implicated in numerous human cancers and may act as a metastasis suppressor gene. Multiple pseudogenes of this gene have been identified in the genome. [provided by RefSeq, Jul 2015]
UniProt Code:P30086
NCBI GenInfo Identifier:1352726
NCBI Gene ID:5037
NCBI Accession:P30086.3
UniProt Secondary Accession:P30086,B2R4S1,
UniProt Related Accession:P30086
Molecular Weight:21,057 Da
NCBI Full Name:Phosphatidylethanolamine-binding protein 1
NCBI Synonym Full Names:phosphatidylethanolamine binding protein 1
NCBI Official Symbol:PEBP1  
NCBI Official Synonym Symbols:PBP; HCNP; PEBP; RKIP; HCNPpp; PEBP-1; HEL-210; HEL-S-34; HEL-S-96  
NCBI Protein Information:phosphatidylethanolamine-binding protein 1
UniProt Protein Name:Phosphatidylethanolamine-binding protein 1
UniProt Synonym Protein Names:HCNPpp; Neuropolypeptide h3; Prostatic-binding protein; Raf kinase inhibitor protein; RKIP
Protein Family:Phosphatidylethanolamine-binding protein
UniProt Gene Name:PEBP1  
UniProt Entry Name:PEBP1_HUMAN
Anti-PEBP1 Antibody (CAB0578)Western blot analysis of extracts of various cell lines, using PEBP1 antibody (CAB0578) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-PEBP1 Antibody (CAB0578)Immunofluorescence analysis of C6 cells using PEBP1 antibody (CAB0578) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-PEBP1 Antibody (CAB0578)Immunofluorescence analysis of L929 cells using PEBP1 antibody (CAB0578) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-PEBP1 Antibody (CAB0578)Immunofluorescence analysis of U-2 OS cells using PEBP1 antibody (CAB0578) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-PEBP1 Antibody (CAB0578)Immunoprecipitation analysis of 150ug extracts of MCF-7 cells using 3ug PEBP1 antibody (CAB0578). Western blot was performed from the immunoprecipitate using PEBP1 antibody (CAB0578) at a dilition of 1:500.
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