Name: Anti-PDZK1 Antibody (CAB5321)
Brand: Cell Biology Antibodies 9
SKU: CAB5321
Availability: OutOfStock

Antibody Name:	Anti-PDZK1 Antibody
Antibody SKU:	CAB5321
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human PDZK1 (NP_001188254.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Mouse
Positive Samples:	Mouse kidney, Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human PDZK1 (NP_001188254.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MTST FNPR ECKL SKQE GQNY GFFL RIEK DTEG HLVR VVEK CSPA EKAG LQDG DRVL RING VFVD KEEH MQVV DLVR KSGN SVTL LVLD GDSY EKAV KTRV DLKE LGQS QKEQ GLSD NILS PVMN GGVQ TWTQ PRLC YLVK EGGS YGFS LKTV QGKK GVYM TDIT PQGV AMRA GVLA DDHL IEVN GENV EDAS HEEV VEKV KKSG SRVM FLLV DKET DKRH VEQK IQFK RETA SLKL LPHQ PRIV EMKK GSNG YGFY LRAG
Gene ID:	5174
Uniprot:	Q5T2W1
Cellular Location:	Cell membrane, Cytoplasm, Membrane, Peripheral membrane protein
Calculated MW:	44kDa/57kDa
Observed MW:	67kDa

Synonyms:

PDZK1, CAP70, CLAMP, NHERF-3, NHERF3, PDZD1

Background:

This gene encodes a PDZ domain-containing scaffolding protein. PDZ domain-containing molecules bind to and mediate the subcellular localization of target proteins. The encoded protein mediates the localization of cell surface proteins and plays a critical role in cholesterol metabolism by regulating the HDL receptor, scavenger receptor class B type 1. Single nucleotide polymorphisms in this gene may be associated with metabolic syndrome, and overexpression of this gene may play a role in drug resistance of multiple myeloma. Pseudogenes of this gene are located on the long arm of chromosome 1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

UniProt Protein Function:	PDZK1: A scaffold protein that connects plasma membrane proteins and regulatory components, regulating their surface expression in epithelial cells apical domains. May be involved in the coordination of a diverse range of regulatory processes for ion transport and second messenger cascades. In complex with SLC9A3R1, may cluster proteins that are functionally dependent in a mutual fashion and modulate the trafficking and the activity of the associated membrane proteins. May play a role in the cellular mechanisms associated with multidrug resistance through its interaction with ABCC2 and PDZK1IP1. May potentiate the CFTR chloride channel activity. May function to connect SCARB1 with the cellular machineries for intracellular cholesterol transport and/or metabolism. May be involved in the regulation of proximal tubular Na(+)-dependent inorganic phosphate cotransport therefore playing an important role in tubule function. Belongs to the NHER family.
UniProt Protein Details:	Protein type:Adaptor/scaffold Chromosomal Location of Human Ortholog: 1q21 Cellular Component: brush border membrane; plasma membrane Molecular Function:PDZ domain binding; protein binding; transporter activity Biological Process: carnitine transport; cell proliferation; transport
NCBI Summary:	This gene encodes a PDZ domain-containing scaffolding protein. PDZ domain-containing molecules bind to and mediate the subcellular localization of target proteins. The encoded protein mediates the localization of cell surface proteins and plays a critical role in cholesterol metabolism by regulating the HDL receptor, scavenger receptor class B type 1. Single nucleotide polymorphisms in this gene may be associated with metabolic syndrome, and overexpression of this gene may play a role in drug resistance of multiple myeloma. Pseudogenes of this gene are located on the long arm of chromosome 1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2011]
UniProt Code:	Q5T2W1
NCBI GenInfo Identifier:	73621372
NCBI Gene ID:	5174
NCBI Accession:	Q5T2W1.2
UniProt Secondary Accession:	Q5T2W1,O60450, Q5T5P6, Q9BQ41, B4DPB9, E7EU02,
UniProt Related Accession:	Q5T2W1
Molecular Weight:	44,665 Da
NCBI Full Name:	Na(+)/H(+) exchange regulatory cofactor NHE-RF3
NCBI Synonym Full Names:	PDZ domain containing 1
NCBI Official Symbol:	PDZK1
NCBI Official Synonym Symbols:	CAP70; CLAMP; PDZD1; NHERF3; NHERF-3
NCBI Protein Information:	Na(+)/H(+) exchange regulatory cofactor NHE-RF3
UniProt Protein Name:	Na(+)/H(+) exchange regulatory cofactor NHE-RF3
UniProt Synonym Protein Names:	CFTR-associated protein of 70 kDa; Na(+)/H(+) exchanger regulatory factor 3; Na/Pi cotransporter C-terminal-associated protein 1; NaPi-Cap1; PDZ domain-containing protein 1; Sodium-hydrogen exchanger regulatory factor 3
Protein Family:	PDZK1-interacting protein
UniProt Gene Name:	PDZK1
UniProt Entry Name:	NHRF3_HUMAN

Western blot analysis of extracts of various cell lines, using PDZK1 antibody (CAB5321) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Antibodies

PDZK1 Antibody (PACO11158)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)