Antibody Name:	Anti-PDYN Antibody
Antibody SKU:	CAB5830
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 21-254 of human PDYN (NP_077722.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-251MG, Mouse liver, Mouse pancreas

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 21-254 of human PDYN (NP_077722.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	DCLS RCSL CAVK TQDG PKPI NPLI CSLQ CQAA LLPS EEWE RCQS FLSF FTPS TLGL NDKE DLGS KSVG EGPY SELA KLSG SFLK ELEK SKFL PSIS TKEN TLSK SLEE KLRG LSDG FREG AESE LMRD AQLN DGAM ETGT LYLA EEDP KEQV KRYG GFLR KYPK RSSE VAGE GDGD SMGH EDLY KRYG GFLR RIRP KLKW DNQK RYGG FLRR QFKV VTRS QEDP NAYS GELF DA
Gene ID:	5173
Uniprot:	P01213
Cellular Location:	Secreted
Calculated MW:	28kDa
Observed MW:	28kDa

Synonyms:

PDYN, ADCA, PENKB, SCA23

Background:

The protein encoded by this gene is a preproprotein that is proteolytically processed to form the secreted opioid peptides beta-neoendorphin, dynorphin, leu-enkephalin, rimorphin, and leumorphin. These peptides are ligands for the kappa-type of opioid receptor. Dynorphin is involved in modulating responses to several psychoactive substances, including cocaine. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.

UniProt Protein Function:	PDYN: Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress. Defects in PDYN are the cause of spinocerebellar ataxia type 23 (SCA23). Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA23 is an adult-onset autosomal dominant form characterized by slowly progressive gait and limb ataxia, with variable additional features, including peripheral neuropathy and dysarthria. Belongs to the opioid neuropeptide precursor family.
UniProt Protein Details:	Protein type:Secreted, signal peptide; Secreted Chromosomal Location of Human Ortholog: 20p13 Cellular Component: plasma membrane; extracellular region Molecular Function:opioid peptide activity Biological Process: synaptic transmission; neuropeptide signaling pathway Disease: Spinocerebellar Ataxia 23
NCBI Summary:	The protein encoded by this gene is a preproprotein that is proteolytically processed to form the secreted opioid peptides beta-neoendorphin, dynorphin, leu-enkephalin, rimorphin, and leumorphin. These peptides are ligands for the kappa-type of opioid receptor. Dynorphin is involved in modulating responses to several psychoactive substances, including cocaine. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2010]
UniProt Code:	P01213
NCBI GenInfo Identifier:	127976
NCBI Gene ID:	5173
NCBI Accession:	P01213.1
UniProt Related Accession:	P01213
Molecular Weight:
NCBI Full Name:	Proenkephalin-B
NCBI Synonym Full Names:	prodynorphin
NCBI Official Symbol:	PDYN
NCBI Official Synonym Symbols:	ADCA; PENKB; SCA23
NCBI Protein Information:	proenkephalin-B
UniProt Protein Name:	Proenkephalin-B
UniProt Synonym Protein Names:	Beta-neoendorphin-dynorphin; PreprodynorphinCleaved into the following 9 chains:Alpha-neoendorphin; Beta-neoendorphin; Big dynorphin; Big Dyn; Dynorphin A(1-17); Dyn-A17; Dynorphin A; Dynorphin A(1-13); Dynorphin A(1-8); Leu-enkephalin; RimorphinAlternative name(s):Dynorphin B; Dyn-B; Dynorphin B(1-13)
Protein Family:	Proenkephalin
UniProt Gene Name:	PDYN
UniProt Entry Name:	PDYN_HUMAN

	Western blot analysis of extracts of various cell lines, using PDYN antibody (CAB5830) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat brain using PDYN antibody (CAB5830) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human lung cancer using PDYN antibody (CAB5830) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human gastric cancer using PDYN antibody (CAB5830) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using PDYN antibody (CAB5830) at dilution of 1:100 (40x lens).