Antibody Name:	Anti-PDP1 Antibody
Antibody SKU:	CAB6332
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 278-537 of human PDP1 (NP_060914.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse brain, Mouse heart, Mouse skeletal muscle, Mouse testis, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 278-537 of human PDP1 (NP_060914.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	HVDG VDLH VANT GDSR AMLG VQEE DGSW SAVT LSND HNAQ NERE LERL KLEH PKSE AKSV VKQD RLLG LLMP FRAF GDVK FKWS IDLQ KRVI ESGP DQLN DNEY TKFI PPNY HTPP YLTA EPEV TYHR LRPQ DKFL VLAT DGLW ETMH RQDV VRIV GEYL TGMH HQQP IAVG GYKV TLGQ MHGL LTER RTKM SSVF EDQN AATH LIRH AVGN NEFG TVDH ERLS KMLS LPEE LARM YRDD ITII VVQF NSHV VGAY QNQE
Gene ID:	54704
Uniprot:	Q9P0J1
Cellular Location:	Mitochondrion matrix
Calculated MW:	61kDa/63kDa
Observed MW:	55kDa

Synonyms:

PDP1, PDH, PDP, PDPC, PPM2A, PPM2C

Background:

Pyruvate dehydrogenase (E1) is one of the three components (E1, E2, and E3) of the large pyruvate dehydrogenase complex. Pyruvate dehydrogenase kinases catalyze phosphorylation of serine residues of E1 to inactivate the E1 component and inhibit the complex. Pyruvate dehydrogenase phosphatases catalyze the dephosphorylation and activation of the E1 component to reverse the effects of pyruvate dehydrogenase kinases. Pyruvate dehydrogenase phosphatase is a heterodimer consisting of catalytic and regulatory subunits. Two catalytic subunits have been reported; one is predominantly expressed in skeletal muscle and another one is is much more abundant in the liver. The catalytic subunit, encoded by this gene, is the former, and belongs to the protein phosphatase 2C (PP2C) superfamily. Along with the pyruvate dehydrogenase complex and pyruvate dehydrogenase kinases, this enzyme is located in the mitochondrial matrix. Mutation in this gene causes pyruvate dehydrogenase phosphatase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been identified.

UniProt Protein Function:	PDP1: a protein phosphoserine phosphatase associated with the mitochondrial matrix that activates phosphorylated pyruvate dehydrogenase complex by dephosphorylation. The PDPs play crucial roles in switching metabolic flux from glycolysis towards oxidative phosphorylation. Catalyzes the dephosphorylation and concomitant reactivation of the alpha subunit of the E1 component of the pyruvate dehydrogenase complex. PDP1 is composed of a catalytic subunit and a regulatory subunit (PDPR). Heterodimer of a catalytic subunit and a FAD protein of unknown function. Defects in PDP1 are the cause of pyruvate dehydrogenase phosphatase deficiency (PDP deficiency). PDP deficiency results in lactic acidosis leading to neurological dysfunction. Belongs to the PP2C family.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; EC 3.1.3.43; Mitochondrial; Protein phosphatase, Ser/Thr (non-receptor) Chromosomal Location of Human Ortholog: 8q22.1 Cellular Component: mitochondrial matrix Molecular Function:[pyruvate dehydrogenase (lipoamide)] phosphatase activity; protein binding; protein serine/threonine phosphatase activity Biological Process: regulation of acetyl-CoA biosynthetic process from pyruvate Disease: Pyruvate Dehydrogenase Phosphatase Deficiency
NCBI Summary:	Pyruvate dehydrogenase (E1) is one of the three components (E1, E2, and E3) of the large pyruvate dehydrogenase complex. Pyruvate dehydrogenase kinases catalyze phosphorylation of serine residues of E1 to inactivate the E1 component and inhibit the complex. Pyruvate dehydrogenase phosphatases catalyze the dephosphorylation and activation of the E1 component to reverse the effects of pyruvate dehydrogenase kinases. Pyruvate dehydrogenase phosphatase is a heterodimer consisting of catalytic and regulatory subunits. Two catalytic subunits have been reported; one is predominantly expressed in skeletal muscle and another one is is much more abundant in the liver. The catalytic subunit, encoded by this gene, is the former, and belongs to the protein phosphatase 2C (PP2C) superfamily. Along with the pyruvate dehydrogenase complex and pyruvate dehydrogenase kinases, this enzyme is located in the mitochondrial matrix. Mutation in this gene causes pyruvate dehydrogenase phosphatase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been identified.[provided by RefSeq, Jun 2009]
UniProt Code:	Q9P0J1
NCBI GenInfo Identifier:	78099789
NCBI Gene ID:	54704
NCBI Accession:	Q9P0J1.3
UniProt Secondary Accession:	Q9P0J1,Q5U5K1, B3KX71, J3KPU0,
UniProt Related Accession:	Q9P0J1
Molecular Weight:	63,695 Da
NCBI Full Name:	[Pyruvate dehydrogenase
NCBI Synonym Full Names:	pyruvate dehyrogenase phosphatase catalytic subunit 1
NCBI Official Symbol:	PDP1
NCBI Official Synonym Symbols:	PDH; PDP; PDPC; PPM2A; PPM2C
NCBI Protein Information:	pyruvate dehyrogenase phosphatase catalytic subunit 1
UniProt Protein Name:	[Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1, mitochondrial
UniProt Synonym Protein Names:	Protein phosphatase 2C; Pyruvate dehydrogenase phosphatase catalytic subunit 1; PDPC 1
Protein Family:	PWWP domain-containing protein
UniProt Gene Name:	PDP1
UniProt Entry Name:	PDP1_HUMAN

	Western blot analysis of extracts of various cell lines, using PDP1 antibody (CAB6332) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded rat brain using PDP1 antibody (CAB6332) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse spinal cord using PDP1 antibody (CAB6332) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using PDP1 antibody (CAB6332) at dilution of 1:100 (40x lens).