Antibody Name:	Anti-PDLIM5 Antibody
Antibody SKU:	CAB5720
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 317-596 of human PDLIM5 (NP_006448.4).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	HL-60, BT-474, HepG2, MCF7, Mouse lung, Mouse heart, Mouse kidney, Mouse uterus

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 317-596 of human PDLIM5 (NP_006448.4).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	ASSV ASTR SMPE SLDS PTSG RPGV TSLT TAAA FKPV GSTG VIKS PSWQ RPNQ GVPS TGRI SNSA TYSG SVAP ANSA LGQT QPSD QDTL VQRA EHIP AGKR TPMC AHCN QVIR GPFL VALG KSWH PEEF NCAH CKNT MAYI GFVE EKGA LYCE LCYE KFFA PECG RCQR KILG EVIS ALKQ TWHV SCFV CVAC GKPI RNNV FHLE DGEP YCET DYYA LFGT ICHG CEFP IEAG DMFL EALG YTWH DTCF VCSV CCES LEGQ TFFS KKDK PLCK KHAH SVNF
Gene ID:	10611
Uniprot:	Q96HC4
Cellular Location:	Cell junction, Cytoplasm, postsynaptic cell membrane, postsynaptic density, synapse, synaptosome
Calculated MW:	15kDa/23kDa/25kDa/52kDa/53kDa/63kDa/67kDa
Observed MW:	68kDa

Synonyms:

PDLIM5, ENH, ENH1, L9, LIM

Background:

This gene encodes a member of a family of proteins that possess a 100-amino acid PDZ domain at the N terminus and one to three LIM domains at the C-terminus. This family member functions as a scaffold protein that tethers protein kinases to the Z-disk in striated muscles. It is thought to function in cardiomyocyte expansion and in restraining postsynaptic growth of excitatory synapses. Alternative splicing of this gene results in multiple transcript variants.

UniProt Protein Function:	LIM: an adaptor protein of the Enigma family. Contains three LIM domains and one PDZ domain. LIM domains are cysteine-rich double zinc fingers composed of 50 to 60 amino acids that are involved in protein-protein interactions. LIM domain-containing proteins are scaffolds for the formation of multiprotein complexes. The proteins are involved in cytoskeleton organization, cell lineage specification, organ development, and oncogenesis. Enigma family proteins possess a 100-amino acid PDZ domain in the N terminus and 1 to 3 LIM domains in the C terminus.
UniProt Protein Details:	Protein type:Adaptor/scaffold Chromosomal Location of Human Ortholog: 4q22 Cellular Component: postsynaptic membrane; neuron projection; membrane; postsynaptic density; cell junction; Z disc; cytosol; actin cytoskeleton Molecular Function:actinin binding; protein kinase C binding; zinc ion binding; protein N-terminus binding; actin binding Biological Process: regulation of synaptogenesis
NCBI Summary:	This gene encodes a member of a family of proteins that possess a 100-amino acid PDZ domain at the N terminus and one to three LIM domains at the C-terminus. This family member functions as a scaffold protein that tethers protein kinases to the Z-disk in striated muscles. It is thought to function in cardiomyocyte expansion and in restraining postsynaptic growth of excitatory synapses. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2012]
UniProt Code:	Q96HC4
NCBI GenInfo Identifier:	317373590
NCBI Gene ID:	10611
NCBI Accession:	Q96HC4.5
UniProt Secondary Accession:	Q96HC4,O60705, Q56VN4, Q5UW38, Q8WVK0, A8K6F9, D6RB78 E9PBF5,
UniProt Related Accession:	Q96HC4
Molecular Weight:	63,945 Da
NCBI Full Name:	PDZ and LIM domain protein 5
NCBI Synonym Full Names:	PDZ and LIM domain 5
NCBI Official Symbol:	PDLIM5
NCBI Official Synonym Symbols:	L9; ENH; LIM; ENH1
NCBI Protein Information:	PDZ and LIM domain protein 5; enigma homolog; enigma-like LIM domain protein; enigma-like PDZ and LIM domains protein
UniProt Protein Name:	PDZ and LIM domain protein 5
UniProt Synonym Protein Names:	Enigma homolog; Enigma-like PDZ and LIM domains protein
UniProt Gene Name:	PDLIM5
UniProt Entry Name:	PDLI5_HUMAN

	Western blot analysis of extracts of various cell lines, using PDLIM5 antibody (CAB5720) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunofluorescence analysis of U2OS cells using PDLIM5 antibody (CAB5720). Blue: DAPI for nuclear staining.