Cell Biology Antibodies 10

Anti-PDLIM3 Antibody (CAB6346)

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SKU:
CAB6346
Product Type:
Antibody
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-PDLIM3 Antibody
Antibody SKU:CAB6346
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 70-230 of human PDLIM3 (NP_001107579.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Mouse
Positive Samples:Mouse lung
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 70-230 of human PDLIM3 (NP_001107579.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:KAAA HQLC LKID RGET HLWS PQVS EDGK AHPF KINL ESEP QEFK PIGT AHNR RAQP FVAA ANID DKRQ VVSA SYNS PIGL YSTS NIQD ALHG QLRG LIPS SPQN EPTA SVPP ESDV YRML HDNR NEPT QPRQ SGSF RVLQ GMVD DGSD DRPA GTRS VRAP V
Gene ID:27295
Uniprot:Q53GG5
Cellular Location:Cytoplasm, Z line, myofibril, sarcomere
Calculated MW:20kDa/34kDa/39kDa
Observed MW:35kDa
Synonyms:PDLIM3, ALP
Background:The protein encoded by this gene contains a PDZ domain and a LIM domain, indicating that it may be involved in cytoskeletal assembly. In support of this, the encoded protein has been shown to bind the spectrin-like repeats of alpha-actinin-2 and to colocalize with alpha-actinin-2 at the Z lines of skeletal muscle. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Aberrant alternative splicing of this gene may play a role in myotonic dystrophy.
UniProt Protein Function:PDLIM3: May play a role in the organization of actin filament arrays within muscle cells. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Cytoskeletal

Chromosomal Location of Human Ortholog: 4q35

Cellular Component: cytoplasm; Z disc; actin cytoskeleton

Molecular Function:protein binding; zinc ion binding; structural constituent of muscle; cytoskeletal protein binding

Biological Process: heart development; actin filament organization

NCBI Summary:The protein encoded by this gene contains a PDZ domain and a LIM domain, indicating that it may be involved in cytoskeletal assembly. In support of this, the encoded protein has been shown to bind the spectrin-like repeats of alpha-actinin-2 and to colocalize with alpha-actinin-2 at the Z lines of skeletal muscle. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Aberrant alternative splicing of this gene may play a role in myotonic dystrophy. [provided by RefSeq, Apr 2012]
UniProt Code:Q53GG5
NCBI GenInfo Identifier:74740479
NCBI Gene ID:27295
NCBI Accession:Q53GG5.1
UniProt Secondary Accession:Q53GG5,O43590, O60439, O60440, Q8N6Y6, Q9BVP4, B2R866
UniProt Related Accession:Q53GG5
Molecular Weight:364
NCBI Full Name:PDZ and LIM domain protein 3
NCBI Synonym Full Names:PDZ and LIM domain 3
NCBI Official Symbol:PDLIM3  
NCBI Official Synonym Symbols:ALP  
NCBI Protein Information:PDZ and LIM domain protein 3; enigma homolog; alpha-actinin-2-associated LIM protein
UniProt Protein Name:PDZ and LIM domain protein 3
UniProt Synonym Protein Names:Actinin-associated LIM protein; Alpha-actinin-2-associated LIM protein
Protein Family:PDZ and LIM domain protein
UniProt Gene Name:PDLIM3  
UniProt Entry Name:PDLI3_HUMAN
Anti-PDLIM3 Antibody (CAB6346)Western blot analysis of extracts of mouse lung, using PDLIM3 antibody (CAB6346) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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