Cell Biology Antibodies 10
Anti-PDLIM1 Antibody (CAB6686)
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- SKU:
- CAB6686
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-PDLIM1 Antibody |
| Antibody SKU: | CAB6686 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-329 of human PDLIM1 (NP_066272.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:10 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | A-549, BT-474, SW480, HepG2, HeLa, Mouse liver, Mouse heart, Mouse lung, Rat lung |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-329 of human PDLIM1 (NP_066272.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MTTQ QIDL QGPG PWGF RLVG GKDF EQPL AISR VTPG SKAA LANL CIGD VITA IDGE NTSN MTHL EAQN RIKG CTDN LTLT VARS EHKV WSPL VTEE GKRH PYKM NLAS EPQE VLHI GSAH NRSA MPFT ASPA SSTT ARVI TNQY NNPA GLYS SENI SNFN NALE SKTA ASGV EANS RPLD HAQP PSSL VIDK ESEV YKML QEKQ ELNE PPKQ STSF LVLQ EILE SEEK GDPN KPSG FRSV KAPV TKVA ASIG NAQK LPMC DKCG TGIV GVFV KLRD RHRH PECY VCTD CGTN LKQK GHFF VEDQ IYCE KHAR ERVT PPEG YEVV TVFP K |
| Gene ID: | 9124 |
| Uniprot: | O00151 |
| Cellular Location: | Cytoplasm, cytoskeleton |
| Calculated MW: | 36kDa |
| Observed MW: | 36kDa |
| Synonyms: | PDLIM1, CLIM1, CLP-36, CLP36, HEL-S-112, hCLIM1 |
| Background: | This gene encodes a member of the enigma protein family. The protein contains two protein interacting domains, a PDZ domain at the amino terminal end and one to three LIM domains at the carboxyl terminal. It is a cytoplasmic protein associated with the cytoskeleton. The protein may function as an adapter to bring other LIM-interacting proteins to the cytoskeleton. Pseudogenes associated with this gene are located on chromosomes 3, 14 and 17. |
| UniProt Protein Function: | CLIM1: a cytoskeletal adaptor protein that bring other proteins to the cytoskeleton. Interacts with alpha-actinins 1, 2 and 4. Recruits the Clik1 kinase to actin stress fibers in nonmuscle cells. Strongly expressed in the heart and skeletal muscle, moderately expressed in the spleen, small intestine, colon, placenta, and lung. A lower level expression is seen in liver, thymus, kidney, prostate and pancreas and is not found in the brain, testis, ovary, and peripheral blood leukocytes. |
| UniProt Protein Details: | Protein type:Cytoskeletal Chromosomal Location of Human Ortholog: 10q23.1 Cellular Component: transcription factor complex; cytoskeleton; focal adhesion; cytoplasm Molecular Function:zinc ion binding; transcription coactivator activity Biological Process: regulation of transcription, DNA-dependent; response to hypoxia; response to oxidative stress |
| NCBI Summary: | This gene encodes a member of the enigma protein family. The protein contains two protein interacting domains, a PDZ domain at the amino terminal end and one to three LIM domains at the carboxyl terminal. It is a cytoplasmic protein associated with the cytoskeleton. The protein may function as an adapter to bring other LIM-interacting proteins to the cytoskeleton. Pseudogenes associated with this gene are located on chromosomes 3, 14 and 17. [provided by RefSeq, Oct 2012] |
| UniProt Code: | O00151 |
| NCBI GenInfo Identifier: | 20178312 |
| NCBI Gene ID: | 9124 |
| NCBI Accession: | O00151.4 |
| UniProt Secondary Accession: | O00151,Q5VZH5, Q9BPZ9, B2RBS6, |
| UniProt Related Accession: | O00151 |
| Molecular Weight: | 329 |
| NCBI Full Name: | PDZ and LIM domain protein 1 |
| NCBI Synonym Full Names: | PDZ and LIM domain 1 |
| NCBI Official Symbol: | PDLIM1 |
| NCBI Official Synonym Symbols: | CLIM1; CLP36; CLP-36; hCLIM1; HEL-S-112 |
| NCBI Protein Information: | PDZ and LIM domain protein 1; elfin; LIM domain protein CLP-36; epididymis secretory protein Li 112; carboxyl terminal LIM domain protein 1 |
| UniProt Protein Name: | PDZ and LIM domain protein 1 |
| UniProt Synonym Protein Names: | C-terminal LIM domain protein 1; Elfin; LIM domain protein CLP-36 |
| Protein Family: | PDZ and LIM domain protein |
| UniProt Gene Name: | PDLIM1 |
| UniProt Entry Name: | PDLI1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PDLIM1 antibody (CAB6686) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunofluorescence analysis of U2OS cells using PDLIM1 antibody (CAB6686). Blue: DAPI for nuclear staining. |
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