Metabolism Antibodies 3

Anti-PDK2 Antibody (CAB4737)

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SKU:
CAB4737
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
PDHK2
Synonyms:
PDKII
Research Area:
Metabolism

Description

Product Name:PDK2 Rabbit mAb
Product Code:CAB4737
Size:20uL, 50uL, 100uL
Synonyms:PDHK2, PDKII
Applications:WB, IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthesized peptide derived from human PDK2
Applications:WB, IHC
Recommended Dilutions:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:Mouse brain, Mouse heart, Rat heart
Immunogen:A synthesized peptide derived from human PDK2
Purification Method:Affinity purification
Storage:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:5164
Uniprot:Q15119
Calculated MW:46kDa
Observed MW:46KDa
UniProt Protein Function:PDHK2: an atypical protein kinase associated with the mitochondrial matrix. The PDHKs play crucial roles in switching metabolic flux from oxidative phosphorylation towards glycolysis. PDHK2 is expressed in all tissues. Contains a HATPase_c catalytic domain, found in several ATP-binding proteins including protein histidine kinases (PHKs), PHDKs, DNA gyrase B, topoisomerases, heat shock proteins, and DNA mismatch repair proteins. PDHK regulates glucose oxidation through inhibitory phosphorylation of the E1 alpha subunit of the mitochondrial pyruvate dehydrogenase complex (PDHC) at any one of 3 inhibitory serine residues. Inhibitory sites 1, 2, and 3 correspond to S293, S300, and S232 in human PDHA1, respectively. Four PDHK isoenzymes have been described, each with different site specificity: all four phosphorylate sites 1 and 2 but at different rates; for site 1 PDHK2 >PDHK4 >PDHK1 >PDHK3; for site 2, PDHK3> PDHK4 > PDHK2 > PDHK1. Only PDHK1 phosphorylates site 3. PDHKs are recruited to the PDHC by binding to a lipoyl group covalently attached to the inner lipoyl domain of the E2 component. PDHA1 deficiency is the most common enzyme defect in patients with primary lactic acidosis. Suppression of PDH by PDHK inhibits the conversion of pyruvate to acetyl-CoA, attenuates mitochondrial respiration, and may contribute to the increased lactate production observed in many tumors. The PDH pathway is repressed in a majority of non-small cell lung carcinomas. Inhibited by AZD7545, dichloroacetate (DCA), and radicicol. Radicicol inhibits kinase activity by binding directly to the ATP-binding pocket of PDHK, similar to HSP90 from the same ATPase/kinase superfamily.
UniProt Protein Details:

Protein type:Protein kinase, atypical; Kinase, protein; Mitochondrial; EC 2.7.11.2; ATYPICAL group; PDHK family; PDHK subfamily

Chromosomal Location of Human Ortholog: 17q21.33

Cellular Component: cytoplasm; mitochondrial matrix; mitochondrial pyruvate dehydrogenase complex; mitochondrion; nucleoplasm

Molecular Function:ATP binding; protein homodimerization activity; protein kinase activity; pyruvate dehydrogenase (acetyl-transferring) kinase activity

Biological Process: cellular response to nutrient; glucose homeostasis; insulin receptor signaling pathway; regulation of acetyl-CoA biosynthetic process from pyruvate; regulation of gluconeogenesis; regulation of pH

NCBI Summary:This gene encodes a member of the pyruvate dehydrogenase kinase family. The encoded protein phosphorylates pyruvate dehydrogenase, down-regulating the activity of the mitochondrial pyruvate dehydrogenase complex. Overexpression of this gene may play a role in both cancer and diabetes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2010]
UniProt Code:Q15119
NCBI GenInfo Identifier:21431820
NCBI Gene ID:5164
NCBI Accession:Q15119.2
UniProt Secondary Accession:Q15119,Q6P515, Q9BS05, A8K3A7, B3KNW0,
UniProt Related Accession:Q15119
Molecular Weight:38,864 Da
NCBI Full Name:[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 2, mitochondrial
NCBI Synonym Full Names:pyruvate dehydrogenase kinase 2
NCBI Official Symbol:PDK2  
NCBI Official Synonym Symbols:PDHK2; PDKII  
NCBI Protein Information:pyruvate dehydrogenase kinase, isozyme 2
UniProt Protein Name:[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 2, mitochondrial
UniProt Synonym Protein Names:Pyruvate dehydrogenase kinase isoform 2; PDH kinase 2; PDKII
UniProt Gene Name:PDK2  
UniProt Entry Name:PDK2_HUMAN
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