Cell Biology Antibodies 14
Anti-PDHA2 Antibody (CAB16944)
- SKU:
- CAB16944
- Product Type:
- Antibody
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-PDHA2 Antibody |
| Antibody SKU: | CAB16944 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of mouse PDHA2 (NP_032837.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Mouse, Rat |
| Positive Samples: | Mouse testis, Rat testis |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of mouse PDHA2 (NP_032837.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | ISYR SREE VHNV RSKS DPIM LLRE RIIS NNLS NIEE LKEI DADV KKEV EDAA QFAT TDPE PAVE DIAN YLYH QDPP FEVR GAHK WLKY KSHS |
| Gene ID: | 18598 |
| Uniprot: | P35487 |
| Cellular Location: | Mitochondrion matrix |
| Calculated MW: | 42kDa |
| Observed MW: | 43kDa |
 | Western blot - PDHA2 Rabbit pAb (CAB16944) |
| Synonyms: | PDHA2, PDHAL, Pdha2 |
| Background: |
| UniProt Protein Function: | PDHA2: a testis-specific mitochondrial matrix enzyme that catalyzes the oxidative decarboxylation of pyruvate, producing acetyl-CoA and CO2. A key enzyme in controlling the balance between lipid and glucose oxidation depending on substrate availability. The pyruvate dehydrogenase (PDH) holoenzyme is a multi-enzyme complex (PDHC) that contains 20-30 copies of pyruvate decarboxylase tetramers (2 alpha:2 beta)(E1), 60 copies of dihydrolipoamide acetyltransferase (E2), six homodimers of dihydrolipoamide dehydrogenase (E3), plus E3 binding proteins. The activity of PDH is tightly regulated by phosphorylation. The phosphorylation of at least one of three specific serine residues in E1 subunit by PDHK inactivates the PDHC, while dephosphorylation by PDP restores its activity. Expressed in postmeiotic spermatogenic cells. |
| UniProt Protein Details: | Protein type:EC 1.2.4.1; Mitochondrial; Carbohydrate Metabolism - butanoate; Carbohydrate Metabolism - citrate (TCA) cycle; Amino Acid Metabolism - valine, leucine and isoleucine biosynthesis; Oxidoreductase; Carbohydrate Metabolism - pyruvate; Carbohydrate Metabolism - glycolysis and gluconeogenesis Cellular Component: intracellular membrane-bound organelle; mitochondrion; pyruvate dehydrogenase complex Molecular Function:oxidoreductase activity; oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor; pyruvate dehydrogenase (acetyl-transferring) activity Biological Process: acetyl-CoA biosynthetic process from pyruvate; carbohydrate metabolic process; glucose metabolic process; metabolic process; tricarboxylic acid cycle |
| UniProt Code: | P35487 |
| NCBI GenInfo Identifier: | 6679263 |
| NCBI Gene ID: | 18598 |
| NCBI Accession: | NP_032837.1 |
| UniProt Secondary Accession: | P35487,Q497M8, |
| UniProt Related Accession: | P35487 |
| Molecular Weight: | 43,413 Da |
| NCBI Full Name: | pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial |
| NCBI Synonym Full Names: | pyruvate dehydrogenase E1 alpha 2 |
| NCBI Official Symbol: | Pdha2 |
| NCBI Official Synonym Symbols: | Pdhal |
| NCBI Protein Information: | pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial |
| UniProt Protein Name: | Pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial |
| UniProt Synonym Protein Names: | PDHE1-A type II |
| Protein Family: | Pyruvate dehydrogenase E1 component |
| UniProt Gene Name: | Pdha2 |
| UniProt Entry Name: | ODPAT_MOUSE |
Related Products
