Metabolism Antibodies 1

Anti-PDF Antibody (CAB10458)

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SKU:
CAB10458
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

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Antibody Name:Anti-PDF Antibody
Antibody SKU:CAB10458
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 40-243 of human PDF (NP_071736.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:MCF7, SW620, A-549, HepG2, K-562, Mouse heart, Rat heart
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 40-243 of human PDF (NP_071736.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:EGPA LRRS YWRH LRRL VLGP PEPP FSHV CQVG DPVL RGVA APVE RAQL GGPE LQRL TQRL VQVM RRRR CVGL SAPQ LGVP RQVL ALEL PEAL CREC PPRQ RALR QMEP FPLR VFVN PSLR VLDS RLVT FPEG CESV AGFL ACVP RFQA VQIS GLDP NGEQ VVWQ ASGW AARI IQHE MDHL QGCL FIDK MDSR TFTN VYWM KVND
Gene ID:64146
Uniprot:Q9HBH1
Cellular Location:Mitochondrion
Calculated MW:27kDa
Observed MW:20kDa
Synonyms:PDF
Background:Protein synthesis proceeds after formylation of methionine by methionyl-tRNA formyl transferase (FMT) and transfer of the charged initiator f-met tRNA to the ribosome. In eubacteria and eukaryotic organelles the product of this gene, peptide deformylase (PDF), removes the formyl group from the initiating methionine of nascent peptides. In eubacteria, deformylation of nascent peptides is required for subsequent cleavage of initiating methionines by methionine aminopeptidase. The discovery that a natural inhibitor of PDF, actinonin, acts as an antimicrobial agent in some bacteria has spurred intensive research into the design of bacterial-specific PDF inhibitors. In human cells, only mitochondrial proteins have N-formylation of initiating methionines. Protein inhibitors of PDF or siRNAs of PDF block the growth of cancer cell lines but have no effect on normal cell growth. In humans, PDF function may therefore be restricted to rapidly growing cells.
UniProt Protein Function:PDF: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Belongs to the polypeptide deformylase family.
UniProt Protein Details:

Protein type:Hydrolase; EC 3.5.1.88; Mitochondrial

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: mitochondrion

Molecular Function:peptide deformylase activity

Biological Process: N-terminal protein amino acid modification; peptidyl-methionine modification; positive regulation of cell proliferation

NCBI Summary:Protein synthesis proceeds after formylation of methionine by methionyl-tRNA formyl transferase (FMT) and transfer of the charged initiator f-met tRNA to the ribosome. In eubacteria and eukaryotic organelles the product of this gene, peptide deformylase (PDF), removes the formyl group from the initiating methionine of nascent peptides. In eubacteria, deformylation of nascent peptides is required for subsequent cleavage of initiating methionines by methionine aminopeptidase. The discovery that a natural inhibitor of PDF, actinonin, acts as an antimicrobial agent in some bacteria has spurred intensive research into the design of bacterial-specific PDF inhibitors. In human cells, only mitochondrial proteins have N-formylation of initiating methionines. Protein inhibitors of PDF or siRNAs of PDF block the growth of cancer cell lines but have no effect on normal cell growth. In humans, PDF function may therefore be restricted to rapidly growing cells. [provided by RefSeq, Nov 2008]
UniProt Code:Q9HBH1
NCBI GenInfo Identifier:17433054
NCBI Gene ID:64146
NCBI Accession:Q9HBH1.1
UniProt Secondary Accession:Q9HBH1,Q8WUN6,
UniProt Related Accession:Q9HBH1
Molecular Weight:27,013 Da
NCBI Full Name:Peptide deformylase, mitochondrial
NCBI Synonym Full Names:peptide deformylase (mitochondrial)
NCBI Official Symbol:PDF  
NCBI Protein Information:peptide deformylase, mitochondrial
UniProt Protein Name:Peptide deformylase, mitochondrial
UniProt Synonym Protein Names:Polypeptide deformylase
Protein Family:Protein
UniProt Gene Name:PDF  
UniProt Entry Name:DEFM_HUMAN
Anti-PDF Antibody (CAB10458)Western blot analysis of extracts of various cell lines, using PDF antibody (CAB10458) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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