Cell Biology Antibodies 2

Anti-PDE8A Antibody (CAB12187)

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SKU:
CAB12187
Product Type:
Antibody
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-PDE8A Antibody
Antibody SKU:CAB12187
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human PDE8A (NP_002596.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Mouse, Rat
Positive Samples:Mouse testis, Mouse eye, Mouse liver, Rat testis
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human PDE8A (NP_002596.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MGCA PSIH ISER LVAE DAPS PAAP PLSS GGPR LPQG QKTA ALPR TRGA GLLE SELR DGSG KKVA VADV QFGP MRFH QDQL QVLL VFTK EDNQ CNGF CRAC EKAG FKCT VTKE AQAV LACF
Gene ID:5151
Uniprot:O60658
Cellular Location:
Calculated MW:25kDa/29kDa/86kDa/88kDa/93kDa
Observed MW:93kDa
Synonyms:PDE8A, HsT19550
Background:The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE8 subfamily. This PDE hydrolyzes the second messenger, cAMP, which is a regulator and mediator of a number of cellular responses to extracellular signals. Thus, by regulating the cellular concentration of cAMP, this protein plays a key role in many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:PDE8A: Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in maintaining basal levels of the cyclic nucleotide and/or in the cAMP regulation of germ cell development. Belongs to the cyclic nucleotide phosphodiesterase family. PDE8 subfamily. 5 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 3.1.4.53; Nucleotide Metabolism - purine; Phosphodiesterase

Chromosomal Location of Human Ortholog: 15q25.3

Cellular Component: cytosol

Molecular Function:3',5'-cyclic-AMP phosphodiesterase activity; kinase binding

Biological Process: positive regulation of protein amino acid phosphorylation

NCBI Summary:The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE8 subfamily. This PDE hydrolyzes the second messenger, cAMP, which is a regulator and mediator of a number of cellular responses to extracellular signals. Thus, by regulating the cellular concentration of cAMP, this protein plays a key role in many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011]
UniProt Code:O60658
NCBI GenInfo Identifier:23503044
NCBI Gene ID:5151
NCBI Accession:O60658.2
UniProt Secondary Accession:O60658,Q6P9H3, Q969I1, Q96PC9, Q96PD0, Q96PD1, Q96T71 Q9UMB7, Q9UMC3, B3KXE6, H0YMZ7,
UniProt Related Accession:O60658
Molecular Weight:93kDa
NCBI Full Name:High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A
NCBI Synonym Full Names:phosphodiesterase 8A
NCBI Official Symbol:PDE8A  
NCBI Official Synonym Symbols:HsT19550  
NCBI Protein Information:high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A
UniProt Protein Name:High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A
Protein Family:High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase
UniProt Gene Name:PDE8A  
Anti-PDE8A Antibody (CAB12187)Western blot analysis of extracts of various cell lines, using PDE8A antibody (CAB12187) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
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