Signal Transduction Antibodies 3

Anti-PDE6A Antibody (CAB7915)

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SKU:
CAB7915
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction

Description

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Antibody Name:Anti-PDE6A Antibody
Antibody SKU:CAB7915
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 656-860 of human PDE6A (NP_000431.2).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:BT-474, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 656-860 of human PDE6A (NP_000431.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:EHAI HMMD IAII ATDL ALYF KKRT MFQK IVDQ SKTY ESEQ EWTQ YMML EQTR KEIV MAMM MTAC DLSA ITKP WEVQ SQVA LLVA AEFW EQGD LERT VLQQ NPIP MMDR NKAD ELPK LQVG FIDF VCTF VYKE FSRF HEEI TPML DGIT NNRK EWKA LADE YDAK MKVQ EEKK QKQQ SAKS AAAG NQPG GNPS PGGA TTSK SCCI Q
Gene ID:5145
Uniprot:P16499
Cellular Location:Cell membrane, Cytoplasmic side, Lipid-anchor
Calculated MW:99kDa
Observed MW:120kDa
Synonyms:PDE6A, CGPR-A, PDEA, RP43
Background:This gene encodes the cyclic-GMP (cGMP)-specific phosphodiesterase 6A alpha subunit, expressed in cells of the retinal rod outer segment. The phosphodiesterase 6 holoenzyme is a heterotrimer composed of an alpha, beta, and two gamma subunits. cGMP is an important regulator of rod cell membrane current, and its dynamic concentration is established by phosphodiesterase 6A cGMP hydrolysis and guanylate cyclase cGMP synthesis. The protein is a subunit of a key phototransduction enzyme and participates in processes of transmission and amplification of the visual signal. Mutations in this gene have been identified as one cause of autosomal recessive retinitis pigmentosa.
UniProt Protein Function:PDE6A: This protein participates in processes of transmission and amplification of the visual signal. Defects in PDE6A are the cause of retinitis pigmentosa type 43 (RP43). RP43 is a retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. Belongs to the cyclic nucleotide phosphodiesterase family.
UniProt Protein Details:

Protein type:EC 3.1.4.35; Phosphodiesterase

Chromosomal Location of Human Ortholog: 5q31.2-q34

Cellular Component: plasma membrane

Molecular Function:3',5'-cyclic-GMP phosphodiesterase activity

Biological Process: cytosolic calcium ion homeostasis; GMP metabolic process; regulation of rhodopsin mediated signaling; rhodopsin mediated signaling; Wnt receptor signaling pathway, calcium modulating pathway

Disease: Retinitis Pigmentosa 43

NCBI Summary:This gene encodes the cyclic-GMP (cGMP)-specific phosphodiesterase 6A alpha subunit, expressed in cells of the retinal rod outer segment. The phosphodiesterase 6 holoenzyme is a heterotrimer composed of an alpha, beta, and two gamma subunits. cGMP is an important regulator of rod cell membrane current, and its dynamic concentration is established by phosphodiesterase 6A cGMP hydrolysis and guanylate cyclase cGMP synthesis. The protein is a subunit of a key phototransduction enzyme and participates in processes of transmission and amplification of the visual signal. Mutations in this gene have been identified as one cause of autosomal recessive retinitis pigmentosa. [provided by RefSeq, Jul 2008]
UniProt Code:P16499
NCBI GenInfo Identifier:215274230
NCBI Gene ID:5145
NCBI Accession:P16499.4
UniProt Secondary Accession:P16499,Q0P638,
UniProt Related Accession:P16499
Molecular Weight:99,547 Da
NCBI Full Name:Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha
NCBI Synonym Full Names:phosphodiesterase 6A
NCBI Official Symbol:PDE6A  
NCBI Official Synonym Symbols:PDEA; RP43; CGPR-A  
NCBI Protein Information:rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha
UniProt Protein Name:Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha
UniProt Synonym Protein Names:PDE V-B1
Protein Family:Rod cGMP-specific 3',5'-cyclic phosphodiesterase
UniProt Gene Name:PDE6A  
UniProt Entry Name:PDE6A_HUMAN
Anti-PDE6A Antibody (CAB7915)Western blot analysis of extracts of various cell lines, using PDE6A antibody (CAB7915) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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