Name: Anti-PDE5A Antibody (CAB6831)
Brand: Cell Biology Antibodies 10
SKU: CAB6831
Availability: OutOfStock

Antibody Name:	Anti-PDE5A Antibody
Antibody SKU:	CAB6831
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human PDE5A (NP_001074.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	HT-1080, SKOV3, A-549, 22Rv1, Mouse brain, Mouse skeletal muscle, Rat skeletal muscle

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human PDE5A (NP_001074.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MERA GPSF GQQR QQQQ PQQQ KQQQ RDQD SVEA WLDD HWDF TFSY FVRK ATRE MVNA WFAE RVHT IPVC KEGI RGHT ESCS CPLQ QSPR ADNS APGT PTRK ISAS EFDR PLRP IVVK DSEG TVSF LSDS EKKE QMPL TPPR FDHD EGDQ CS
Gene ID:	8654
Uniprot:	O76074
Cellular Location:
Calculated MW:	94kDa/99kDa
Observed MW:	100kDa

Synonyms:

PDE5A, CGB-PDE, CN5A, PDE5

Background:

This gene encodes a cGMP-binding, cGMP-specific phosphodiesterase, a member of the cyclic nucleotide phosphodiesterase family. This phosphodiesterase specifically hydrolyzes cGMP to 5'-GMP. It is involved in the regulation of intracellular concentrations of cyclic nucleotides and is important for smooth muscle relaxation in the cardiovascular system. Alternative splicing of this gene results in three transcript variants encoding distinct isoforms.

UniProt Protein Function:	PDE5A: a cGMP-binding, cGMP-specific phosphodiesterase, a member of the cyclic nucleotide phosphodiesterase family. This phosphodiesterase specifically hydrolyzes cGMP to 5'-GMP. It is involved in the regulation of intracellular concentrations of cyclic nucleotides and is important for smooth muscle relaxation in the cardiovascular system. Phosphorylation is regulated by binding of cGMP to the two allosteric sites. Sildenafil (Viagra) is a highly selective and potent inhibitor of PDE5A. Alternative splicing results in four transcript variants encoding distinct isoforms.
UniProt Protein Details:	Protein type:Phosphodiesterase; Nucleotide Metabolism - purine; EC 3.1.4.35 Chromosomal Location of Human Ortholog: 4q27 Cellular Component: cytosol Molecular Function:3',5'-cyclic-GMP phosphodiesterase activity; 3',5'-cyclic-nucleotide phosphodiesterase activity; cGMP binding; metal ion binding Biological Process: cGMP catabolic process; negative regulation of T cell proliferation; positive regulation of MAP kinase activity; regulation of cGMP metabolic process; signal transduction
NCBI Summary:	This gene encodes a cGMP-binding, cGMP-specific phosphodiesterase, a member of the cyclic nucleotide phosphodiesterase family. This phosphodiesterase specifically hydrolyzes cGMP to 5'-GMP. It is involved in the regulation of intracellular concentrations of cyclic nucleotides and is important for smooth muscle relaxation in the cardiovascular system. Alternative splicing of this gene results in three transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]
UniProt Code:	O76074
NCBI GenInfo Identifier:	317373261
NCBI Gene ID:	8654
NCBI Accession:	O76074.2
UniProt Secondary Accession:	O76074,O75026, O75887, Q86UI0, Q86V66, Q9Y6Z6, A0AV69 A8K2C4,
UniProt Related Accession:	O76074
Molecular Weight:	94,805 Da
NCBI Full Name:	cGMP-specific 3',5'-cyclic phosphodiesterase
NCBI Synonym Full Names:	phosphodiesterase 5A
NCBI Official Symbol:	PDE5A
NCBI Official Synonym Symbols:	CN5A; PDE5; CGB-PDE
NCBI Protein Information:	cGMP-specific 3',5'-cyclic phosphodiesterase
UniProt Protein Name:	cGMP-specific 3',5'-cyclic phosphodiesterase
UniProt Synonym Protein Names:	cGMP-binding cGMP-specific phosphodiesterase; CGB-PDE
UniProt Gene Name:	PDE5A
UniProt Entry Name:	PDE5A_HUMAN

Western blot analysis of extracts of various cell lines, using PDE5A antibody (CAB6831) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.

ELISA Kits

Human PDE5A / cGMP-specific 3',5'-cyclic phosphodiesterase ELISA Kit

Human cGMP-specific 3',5'-cyclic phosphodiesterase (PDE5A) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)