Antibody Name:	Anti-PDE1B Antibody
Antibody SKU:	CAB2102
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 190-536 of human PDE1B (NP_000915.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	NCI-H460, THP-1, Mouse brain, Mouse spleen, Mouse spinal cord

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 190-536 of human PDE1B (NP_000915.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	RHNL ISRF KIPT VFLM SFLD ALET GYGK YKNP YHNQ IHAA DVTQ TVHC FLLR TGMV HCLS EIEL LAII FAAA IHDY EHTG TTNS FHIQ TKSE CAIV YNDR SVLE NHHI SSVF RLMQ DDEM NIFI NLTK DEFV ELRA LVIE MVLA TDMS CHFQ QVKT MKTA LQQL ERID KPKA LSLL LHAA DISH PTKQ WLVH SRWT KALM EEFF RQGD KEAE LGLP FSPL CDRT STLV AQSQ IGFI DFIV EPTF SVLT DVAE KSVQ PLAD EDSK SKNQ PSFQ WRQP SLDV EVGD PNPD VVSF RSTW VKRI QENK QKWK ERAA SGIT NQMS IDEL SPCE EEAP PSPA EDEH NQNG NLD
Gene ID:	5153
Uniprot:	Q01064
Cellular Location:	Cytoplasm
Calculated MW:	59kDa/61kDa
Observed MW:	59kDa

Synonyms:

PDE1B, HEL-S-79p, PDE1B1, PDES1B

Background:

The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE1 subfamily. Members of the PDE1 family are calmodulin-dependent PDEs that are stimulated by a calcium-calmodulin complex. This PDE has dual-specificity for the second messengers, cAMP and cGMP, with a preference for cGMP as a substrate. cAMP and cGMP function as key regulators of many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.

UniProt Protein Function:	PDE1B: Cyclic nucleotide phosphodiesterase with a dual- specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Has a preference for cGMP as a substrate. Belongs to the cyclic nucleotide phosphodiesterase family. PDE1 subfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:EC 3.1.4.17; Nucleotide Metabolism - purine; Phosphodiesterase; Apoptosis Chromosomal Location of Human Ortholog: 12q13 Cellular Component: cell soma; cytosol Molecular Function:calmodulin binding; 3',5'-cyclic-AMP phosphodiesterase activity; calmodulin-dependent cyclic-nucleotide phosphodiesterase activity; calcium- and calmodulin-regulated 3',5'-cyclic-GMP phosphodiesterase activity; metal ion binding Biological Process: epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; monocyte differentiation; apoptosis; regulation of neurotransmitter levels; response to amphetamine; cAMP catabolic process; regulation of dopamine metabolic process; locomotory behavior; signal transduction; phospholipase C activation; innate immune response; visual learning; cGMP catabolic process; blood coagulation; serotonin metabolic process
NCBI Summary:	The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE1 subfamily. Members of the PDE1 family are calmodulin-dependent PDEs that are stimulated by a calcium-calmodulin complex. This PDE has dual-specificity for the second messengers, cAMP and cGMP, with a preference for cGMP as a substrate. cAMP and cGMP function as key regulators of many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011]
UniProt Code:	Q01064
NCBI GenInfo Identifier:	3183514
NCBI Gene ID:	5153
NCBI Accession:	Q01064.2
UniProt Related Accession:	Q01064
Molecular Weight:	59kDa
NCBI Full Name:	Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B
NCBI Synonym Full Names:	phosphodiesterase 1B
NCBI Official Symbol:	PDE1B
NCBI Official Synonym Symbols:	PDE1B1; PDES1B; HEL-S-79p
NCBI Protein Information:	calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B
UniProt Protein Name:	Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B
UniProt Synonym Protein Names:	63 kDa Cam-PDE
UniProt Gene Name:	PDE1B
UniProt Entry Name:	PDE1B_HUMAN

	Western blot analysis of extracts of various cell lines, using PDE1B antibody (CAB2102) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded rat brain using PDE1B antibody (CAB2102) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using PDE1B antibody (CAB2102). Blue: DAPI for nuclear staining.