KO Validated Antibodies 1

Anti-PDCD4 Antibody (CAB2570)[KO Validated]

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SKU:
CAB2570
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Death

Description

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Antibody Name:Anti-PDCD4 Antibody [KO Validated]
Antibody SKU:CAB2570
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human PDCD4 (NP_055271.2).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:MCF-7, HeLa, HepG2, Mouse thymus
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human PDCD4 (NP_055271.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MDVE NEQI LNVN PADP DNLS DSLF SGDE ENAG TEEI KNEI NGNW ISAS SINE ARIN AKAK RRLR KNSS RDSG RGDS VSDS GSDA LRSG LTVP TSPK GRLL DRRS RSGK GRGL PKKG GAGG KGVW GTPG QVYD VEEV DVKD PNYD DDQE NCVY ETVV LPLD ERAF EKTL TPII QEYF EHGD TNEV AEML RDLN LGEM KSGV PVLA VSLA LEGK ASHR EMTS KLLS DLCG TVMS TTDV EKSF DKLL KDLP ELAL DTPR APQL
Gene ID:27250
Uniprot:Q53EL6
Cellular Location:Cytoplasm, Nucleus
Calculated MW:50kDa/51kDa
Observed MW:60kDa
Synonyms:PDCD4, H731
Background:This gene is a tumor suppressor and encodes a protein that binds to the eukaryotic translation initiation factor 4A1 and inhibits its function by preventing RNA binding. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:PDCD4: protein localized to the nucleus in proliferating cells that seems to possess a tumor suppressor activity. Directly interacts with the RNA helicase eIF4A and inhibits protein synthesis by interfering with the assembly of the cap-dependent translation initiation complex. Suppresses carbonic anhydrase type II protein expression in carcinoid cell lines. Since tumor cells require a high bicarbonate flux for their growth, carbonic anhydrase suppression results in growth inhibition. Expression of this gene is modulated by cytokines in natural killer and T cells. The gene product is thought to play a role in apoptosis but the specific role has not yet been determined. Two differentially spliced isoforms have been identified.
UniProt Protein Details:

Protein type:Tumor suppressor; Apoptosis

Chromosomal Location of Human Ortholog: 10q24

Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus

Molecular Function:protein binding; RNA binding

Biological Process: apoptosis; cell aging; negative regulation of apoptosis; negative regulation of cell cycle; negative regulation of JNK activity; negative regulation of transcription, DNA-dependent

NCBI Summary:This gene is a tumor suppressor and encodes a protein that binds to the eukaryotic translation initiation factor 4A1 and inhibits its function by preventing RNA binding. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2010]
UniProt Code:Q53EL6
NCBI GenInfo Identifier:317373317
NCBI Gene ID:27250
NCBI Accession:Q53EL6.2
UniProt Secondary Accession:Q53EL6,O15501, Q5VZS6, Q6PJI5, Q8TAR5, Q99834, B2RCV4 B5ME91,
UniProt Related Accession:Q53EL6
Molecular Weight:50,576 Da
NCBI Full Name:Programmed cell death protein 4
NCBI Synonym Full Names:programmed cell death 4 (neoplastic transformation inhibitor)
NCBI Official Symbol:PDCD4  
NCBI Official Synonym Symbols:H731  
NCBI Protein Information:programmed cell death protein 4
UniProt Protein Name:Programmed cell death protein 4
UniProt Synonym Protein Names:Neoplastic transformation inhibitor protein; Nuclear antigen H731-like; Protein 197/15a
Protein Family:Programmed cell death protein
UniProt Gene Name:PDCD4  
UniProt Entry Name:PDCD4_HUMAN
Anti-PDCD4 Antibody [KO Validated] (CAB2570)Western blot analysis of extracts of various cell lines, using PDCD4 antibody (CAB2570) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
Anti-PDCD4 Antibody [KO Validated] (CAB2570)Western blot analysis of extracts from normal (control) and PDCD4 knockout (KO) HeLa cells, using PDCD4 antibody (CAB2570) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-PDCD4 Antibody [KO Validated] (CAB2570)Immunohistochemistry of paraffin-embedded human thyroid cancer using [KO Validated] PDCD4 Rabbit pAb (CAB2570) at dilution of 1:25 (40x lens).
Anti-PDCD4 Antibody [KO Validated] (CAB2570)Immunohistochemistry of paraffin-embedded human placenta using [KO Validated] PDCD4 Rabbit pAb (CAB2570) at dilution of 1:25 (40x lens).
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