KO Validated Antibodies 2

Anti-PD-L1 Antibody [KO Validated] (CAB19135)

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SKU:
CAB19135
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Immunology

Description

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Antibody Name:Anti-PD-L1 Antibody [KO Validated]
Antibody SKU:CAB19135
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide of human PD-L1.
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Rat
Positive Samples:A-549
Immunogen:A synthetic peptide of human PD-L1.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:29126
Uniprot:Q9NZQ7
Cellular Location:Cell membrane, Endomembrane system, Single-pass type I membrane protein
Calculated MW:20kDa/33kDa
Observed MW:43-50kDa
Anti-PD-L1 Antibody [KO Validated] (CAB19135)Western blot analysis of extracts from normal (control) and PD-L1 knockout (KO) A-549 cells, using PD-L1 antibody (CAB19135) at 1:1000 dilution.Both A-549 cells and PD-L1 knockout (KO) A-549 cells were treated by IFNG (100 ng/mL) at 37'C for 48 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-PD-L1 Antibody [KO Validated] (CAB19135)Immunohistochemistry of paraffin-embedded human placenta using [KO Validated] PD-L1 Rabbit mAb (CAB19135) at dilution of 1:100 (40x lens).
Synonyms:B7-H, B7H1, PDL1, PD-L1, PDCD1L1, PDCD1LG1, CD274
Background:This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:CD274: Involved in the costimulatory signal, essential for T- cell proliferation and production of IL10 and IFNG, in an IL2- dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production. Belongs to the immunoglobulin superfamily. BTN/MOG family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Membrane protein, integral

Chromosomal Location of Human Ortholog: 9p24.1

Cellular Component: plasma membrane

Molecular Function:protein binding

Biological Process: cell surface receptor linked signal transduction; immune response; negative regulation of activated T cell proliferation; negative regulation of interferon-gamma production; negative regulation of interleukin-10 production; positive regulation of T cell proliferation; regulation of activated T cell proliferation; response to cytokine stimulus; signal transduction; T cell costimulation

NCBI Summary:This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
UniProt Code:Q9NZQ7
NCBI GenInfo Identifier:83287884
NCBI Gene ID:29126
NCBI Accession:Q9NZQ7.1
UniProt Secondary Accession:Q9NZQ7,Q14CJ2, Q2V8D5, Q66RK1, Q6WEX4, Q9NUZ5, B2RBA2 B4DU27,
UniProt Related Accession:Q9NZQ7
Molecular Weight:20,454 Da
NCBI Full Name:Programmed cell death 1 ligand 1
NCBI Synonym Full Names:CD274 molecule
NCBI Official Symbol:CD274  
NCBI Official Synonym Symbols:B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1  
NCBI Protein Information:programmed cell death 1 ligand 1
UniProt Protein Name:Programmed cell death 1 ligand 1
UniProt Synonym Protein Names:B7 homolog 1; B7-H1; CD_antigen: CD274
UniProt Gene Name:CD274  
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