Metabolism Antibodies 1
Anti-PCYT2 Antibody (CAB15309)
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- SKU:
- CAB15309
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-PCYT2 Antibody |
| Antibody SKU: | CAB15309 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-389 of human PCYT2 (NP_002852.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:200 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | MCF7, HepG2, 293T, Mouse brain, Mouse liver, Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-389 of human PCYT2 (NP_002852.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MIRN GRGA AGGA EQPG PGGR RAVR VWCD GCYD MVHY GHSN QLRQ ARAM GDYL IVGV HTDE EIAK HKGP PVFT QEER YKMV QAIK WVDE VVPA APYV TTLE TLDK YNCD FCVH GNDI TLTV DGRD TYEE VKQA GRYR ECKR TQGV STTD LVGR MLLV TKAH HSSQ EMSS EYRE YADS FGKC PGGR NPWT GVSQ FLQT SQKI IQFA SGKE PQPG ETVI YVAG AFDL FHIG HVDF LEKV HRLA ERPY IIAG LHFD QEVN HYKG KNYP IMNL HERT LSVL ACRY VSEV VIGA PYAV TAEL LSHF KVDL VCHG KTEI IPDR DGSD PYQE PKRR GIFR QIDS GSNL TTDL IVQR IITN RLEY EARN QKKE AKEL AFLE AARQ QAAQ PLGE RDGD F |
| Gene ID: | 5833 |
| Uniprot: | Q99447 |
| Cellular Location: | |
| Calculated MW: | 35kDa/40kDa/43kDa/45kDa |
| Observed MW: | 44kDa |
| Synonyms: | PCYT2, ET |
| Background: | This gene encodes an enzyme that catalyzes the formation of CDP-ethanolamine from CTP and phosphoethanolamine in the Kennedy pathway of phospholipid synthesis. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | Plays an important role in the biosynthesis of the phospholipid phosphatidylethanolamine. Catalyzes the formation of CDP-ethanolamine. |
| NCBI Summary: | This gene encodes an enzyme that catalyzes the formation of CDP-ethanolamine from CTP and phosphoethanolamine in the Kennedy pathway of phospholipid synthesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2010] |
| UniProt Code: | Q99447 |
| NCBI GenInfo Identifier: | 14603223 |
| NCBI Gene ID: | 5833 |
| NCBI Accession: | AAH10075.1 |
| UniProt Secondary Accession: | Q99447,Q6IBM3, Q96G08, B7Z7A5, B7ZAS0, F5H8B1, |
| UniProt Related Accession: | Q99447 |
| Molecular Weight: | 35,200 Da |
| NCBI Full Name: | Phosphate cytidylyltransferase 2, ethanolamine |
| NCBI Synonym Full Names: | phosphate cytidylyltransferase 2, ethanolamine |
| NCBI Official Symbol: | PCYT2 |
| NCBI Official Synonym Symbols: | ET |
| NCBI Protein Information: | ethanolamine-phosphate cytidylyltransferase |
| UniProt Protein Name: | Ethanolamine-phosphate cytidylyltransferase |
| UniProt Synonym Protein Names: | CTP:phosphoethanolamine cytidylyltransferase; Phosphorylethanolamine transferase |
| UniProt Gene Name: | PCYT2 |
 | Western blot analysis of extracts of various cell lines, using PCYT2 antibody (CAB15309) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
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