Cell Cycle Antibodies 1

Anti-PCNP Antibody (CAB15482)

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SKU:
CAB15482
Product Type:
Antibody
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle

Description

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Antibody Name:Anti-PCNP Antibody
Antibody SKU:CAB15482
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-178 of human PCNP (NP_065090.1).
Application:WB
Recommended Dilution:WB 1:200 - 1:2000
Reactivity:Mouse, Rat
Positive Samples:Mouse liver, Rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-178 of human PCNP (NP_065090.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MADG KAGD EKPE KSQR AGAA GGPE EEAE KPVK TKTV SSSN GGES SSRS AEKR SAEE EAAD LPTK PTKI SKFG FAIG SQTT KKAS AISI KLGS SKPK ETVP TLAP KTLS VAAA FNED EDSE PEEM PPEA KMRM KNIG RDTP TSAG PNSF NKGK HGFS DNQK LWER NIKS HLGN VHDQ DN
Gene ID:57092
Uniprot:Q8WW12
Cellular Location:Nucleus
Calculated MW:6kDa/13kDa/18kDa
Observed MW:26kDa
Synonyms:PCNP
Background:
UniProt Protein Function:PCNP: a nuclear protein that may be involved in cell cycle regulation. Ubiquitination by UHRF2 leads to its subsequent proteasomal degradation. Three alternatively spliced isoforms have been reported.
UniProt Protein Details:

Protein type:Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 3q12.3

Cellular Component: nucleoplasm; nucleus

Molecular Function:protein binding

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; protein ubiquitination; cell cycle

UniProt Code:Q8WW12
NCBI GenInfo Identifier:9966826
NCBI Gene ID:57092
NCBI Accession:NP_065090.1
UniProt Secondary Accession:Q8WW12,Q53GF3, Q6AI44, Q96CU3, Q9NS81, B2RBE7, D3DN52
UniProt Related Accession:Q8WW12
Molecular Weight:6,233 Da
NCBI Full Name:Homo sapiens PEST proteolytic signal containing nuclear protein (PCNP), mRNA
NCBI Synonym Full Names:PEST proteolytic signal containing nuclear protein
NCBI Official Symbol:PCNP  
NCBI Protein Information:PEST proteolytic signal-containing nuclear protein; PEST-containing nuclear protein
UniProt Protein Name:PEST proteolytic signal-containing nuclear protein
Protein Family:PEST proteolytic signal-containing nuclear protein
UniProt Gene Name:PCNP  
UniProt Entry Name:PCNP_HUMAN
Anti-PCNP Antibody (CAB15482)Western blot analysis of extracts of various cell lines, using PCNP antibody (CAB15482) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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