Antibody Name:	Anti-PCMT1 Antibody
Antibody SKU:	CAB6684
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 62-286 of human PCMT1 (NP_001238978.1).

Application:	WB IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100 IP 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF7, HepG2, 293T, A-431, Mouse liver, Mouse brain, Rat brain, Rat heart, Rat heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 62-286 of human PCMT1 (NP_001238978.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	KSGG ASHS ELIH NLRK NGII KTDK VFEV MLAT DRSH YAKC NPYM DSPQ SIGF QATI SAPH MHAY ALEL LFDQ LHEG AKAL DVGS GSGI LTAC FARM VGCT GKVI GIDH IKEL VDDS VNNV RKDD PTLL SSGR VQLV VGDG RMGY AEEA PYDA IHVG AAAP VVPQ ALID QLKP GGRL ILPV GPAG GNQM LEQY DKLQ DGSI KMKP LMGV IYVP LTDK EKQW SRDE L
Gene ID:	5110
Uniprot:	P22061
Cellular Location:	Cytoplasm
Calculated MW:	24kDa
Observed MW:	25kDa

Synonyms:

PCMT1, PIMT

Background:

This gene encodes a member of the type II class of protein carboxyl methyltransferase enzymes. The encoded enzyme plays a role in protein repair by recognizing and converting D-aspartyl and L-isoaspartyl residues resulting from spontaneous deamidation back to the normal L-aspartyl form. The encoded protein may play a protective role in the pathogenesis of Alzheimer's disease, and single nucleotide polymorphisms in this gene have been associated with spina bifida and premature ovarian failure. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

UniProt Protein Function:	PCMT1: Catalyzes the methyl esterification of L-isoaspartyl and D-aspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins. Acts on microtubule-associated protein 2, calreticulin, clathrin light chains a and b, Ubiquitin carboxyl- terminal hydrolase isozyme L1, phosphatidylethanolamine-binding protein 1, stathmin, beta-synuclein and alpha-synuclein. Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:EC 2.1.1.77; Methyltransferase Chromosomal Location of Human Ortholog: 6q25.1 Cellular Component: cytoplasm; cytosol; endoplasmic reticulum Molecular Function:protein binding; protein-L-isoaspartate (D-aspartate) O-methyltransferase activity Biological Process: protein amino acid methylation; protein repair
NCBI Summary:	This gene encodes a member of the type II class of protein carboxyl methyltransferase enzymes. The encoded enzyme plays a role in protein repair by recognizing and converting D-aspartyl and L-isoaspartyl residues resulting from spontaneous deamidation back to the normal L-aspartyl form. The encoded protein may play a protective role in the pathogenesis of Alzheimer's disease, and single nucleotide polymorphisms in this gene have been associated with spina bifida and premature ovarian failure. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Oct 2011]
UniProt Code:	P22061
NCBI GenInfo Identifier:	317373537
NCBI Gene ID:	5110
NCBI Accession:	P22061.4
UniProt Secondary Accession:	P22061,Q14661, Q16556, Q5VYC1, Q5VYC2, Q93061, Q96II9 Q99625, Q9BQV7, Q9BQV8, A8K109, J3KP72,
UniProt Related Accession:	P22061
Molecular Weight:	24636 Da
NCBI Full Name:	Protein-L-isoaspartate(D-aspartate) O-methyltransferase
NCBI Synonym Full Names:	protein-L-isoaspartate (D-aspartate) O-methyltransferase
NCBI Official Symbol:	PCMT1
NCBI Official Synonym Symbols:	PIMT
NCBI Protein Information:	protein-L-isoaspartate(D-aspartate) O-methyltransferase
UniProt Protein Name:	Protein-L-isoaspartate(D-aspartate) O-methyltransferase
UniProt Synonym Protein Names:	L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl/D-aspartyl methyltransferase; Protein-beta-aspartate methyltransferase
UniProt Gene Name:	PCMT1
UniProt Entry Name:	PIMT_HUMAN

	Western blot analysis of extracts of various cell lines, using PCMT1 antibody (CAB6684). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunofluorescence analysis of HeLa cells using PCMT1 antibody (CAB6684). Blue: DAPI for nuclear staining.
	Immunoprecipitation analysis of 200ug extracts of HepG2 cells using 1ug PCMT1 antibody (CAB6684). Western blot was performed from the immunoprecipitate using PCMT1 antibody (CAB6684) at a dilition of 1:1000.