Antibody Name:	Anti-PCGF6 Antibody
Antibody SKU:	CAB5760
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 161-350 of human PCGF6 (NP_001011663.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF7

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 161-350 of human PCGF6 (NP_001011663.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	RHFY YSNR CPKC NIVV HQTQ PLYN IRLD RQLQ DIVY KLVI NLEE REKK QMHD FYKE RGLE VPKP AVPQ PVPS SKGR SKKV LESV FRIP PELD MSLL LEFI GANE GTGH FKPL EKKF VRVS GEAT IGHV EKFL RRKM GLDP ACQV DIIC GDHL LEQY QTLR EIRR AIGD AAMQ DGLL VLHY GLVV SPLK IT
Gene ID:	84108
Uniprot:	Q9BYE7
Cellular Location:	Nucleus
Calculated MW:	30kDa/39kDa
Observed MW:	39kDa

Synonyms:

PCGF6, MBLR, RNF134

Background:

The protein encoded by this gene contains a RING finger motif, which is most closely related to those of polycomb group (PcG) proteins RNF110/MEL-18 and BMI1. PcG proteins are known to form protein complexes and function as transcription repressors. This protein has been shown to interact with some PcG proteins and act as a transcription repressor. The activity of this protein is found to be regulated by cell cycle dependent phosphorylation. Alternatively spliced transcript variants encoding different isoforms have been identified.

UniProt Protein Function:	MBLR: Transcriptional repressor. May modulate the levels of histone H3K4Me3 by activating KDM5D histone demethylase. Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Ubiquitin conjugating system Chromosomal Location of Human Ortholog: 10q24.33 Cellular Component: nucleus; PcG protein complex Molecular Function:DNA binding; protein binding; transcription factor activity Biological Process: negative regulation of transcription, DNA-dependent
NCBI Summary:	The protein encoded by this gene contains a RING finger motif, which is most closely related to those of polycomb group (PcG) proteins RNF110/MEL-18 and BMI1. PcG proteins are known to form protein complexes and function as transcription repressors. This protein has been shown to interact with some PcG proteins and act as a transcription repressor. The activity of this protein is found to be regulated by cell cycle dependent phosphorylation. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9BYE7
NCBI GenInfo Identifier:	116242703
NCBI Gene ID:	84108
NCBI Accession:	Q9BYE7.2
UniProt Secondary Accession:	Q9BYE7,Q5SYD1, Q5SYD6, Q96ID9, Q96SJ1, A8K3R4,
UniProt Related Accession:	Q9BYE7
Molecular Weight:	30,249 Da
NCBI Full Name:	Polycomb group RING finger protein 6
NCBI Synonym Full Names:	polycomb group ring finger 6
NCBI Official Symbol:	PCGF6
NCBI Official Synonym Symbols:	MBLR; RNF134
NCBI Protein Information:	polycomb group RING finger protein 6
UniProt Protein Name:	Polycomb group RING finger protein 6
UniProt Synonym Protein Names:	Mel18 and Bmi1-like RING finger; RING finger protein 134
Protein Family:	Polycomb group RING finger protein
UniProt Gene Name:	PCGF6
UniProt Entry Name:	PCGF6_HUMAN

	Western blot analysis of extracts of MCF7 cells, using PCGF6 antibody (CAB5760) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 120s.
	Immunofluorescence analysis of U2OS cells using PCGF6 antibody (CAB5760).