Name: Anti-PCGF1 Antibody (CAB6338)
Brand: Epigenetics & Nuclear Signaling Antibodies 2
SKU: CAB6338
Availability: OutOfStock

Antibody Name:	Anti-PCGF1 Antibody
Antibody SKU:	CAB6338
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 20-259 of human PCGF1 (NP_116062.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	Human fetal brain, HeLa, MCF7, Jurkat

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 20-259 of human PCGF1 (NP_116062.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	QSVY KMDP LRNE EEVR VKIK DLNE HIVC CLCA GYFV DATT ITEC LHTF CKSC IVKY LQTS KYCP MCNI KIHE TQPL LNLK LDRV MQDI VYKL VPGL QDSE EKRI REFY QSRG LDRV TQPT GEEP ALSN LGLP FSSF DHSK AHYY RYDE QLNL CLER LSSG KDKN KSVL QNKY VRCS VRAE VRHL RRVL CHRL MLNP QHVQ LLFD NEVL PDHM TMKQ IWLS RWFG KPSP LLLQ YSVK EKRR
Gene ID:	84759
Uniprot:	Q9BSM1
Cellular Location:	Nucleus
Calculated MW:	20kDa/30kDa
Observed MW:	30kDa

Synonyms:	PCGF1, 2010002K04Rik, NSPC1, RNF3A-2, RNF68
Background:

UniProt Protein Function:	PCGF1: Component of the Polycomb group (PcG) multiprotein BCOR complex, a complex required to maintain the transcriptionally repressive state of some genes, such as BCL6 and the cyclin- dependent kinase inhibitor, CDKN1A. Transcriptional repressor that may be targeted to the DNA by BCL6; this transcription repressor activity may be related to PKC signaling pathway. Represses CDKN1A expression by binding to its promoter, and this repression is dependent on the retinoic acid response element (RARE element). Promotes cell cycle progression and enhances cell proliferation as well. May have a positive role in tumor cell growth by down- regulating CDKN1A. Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:EC 6.3.2.-; Ubiquitin ligase; Ligase; Ubiquitin conjugating system Chromosomal Location of Human Ortholog: 2p13.1 Cellular Component: nucleus; PcG protein complex Molecular Function:protein binding; protein C-terminus binding Biological Process: regulation of transcription, DNA-dependent
NCBI Summary:	PCGF1 is a mammalian homolog of the Drosophila polycomb group genes, which act as transcriptional repressors to regulate anterior-posterior patterning in early embryonic development (Nunes et al., 2001 [PubMed 11287196]). See also PCGF2 (MIM 600346).[supplied by OMIM, Aug 2008]
UniProt Code:	Q9BSM1
NCBI GenInfo Identifier:	223590124
NCBI Gene ID:	84759
NCBI Accession:	Q9BSM1.2
UniProt Secondary Accession:	Q9BSM1,Q7Z506,
UniProt Related Accession:	Q9BSM1
Molecular Weight:	20,904 Da
NCBI Full Name:	Polycomb group RING finger protein 1
NCBI Synonym Full Names:	polycomb group ring finger 1
NCBI Official Symbol:	PCGF1
NCBI Official Synonym Symbols:	NSPC1; RNF68; RNF3A-2; 2010002K04Rik
NCBI Protein Information:	polycomb group RING finger protein 1
UniProt Protein Name:	Polycomb group RING finger protein 1
UniProt Synonym Protein Names:	Nervous system Polycomb-1; NSPc1; RING finger protein 68
Protein Family:	Polycomb group RING finger protein
UniProt Gene Name:	PCGF1
UniProt Entry Name:	PCGF1_HUMAN

	Western blot analysis of extracts of various cell lines, using PCGF1 antibody (CAB6338). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunofluorescence analysis of L-929 cells using PCGF1 antibody (CAB6338) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U-2OS cells using PCGF1 antibody (CAB6338) at dilution of 1:100. Blue: DAPI for nuclear staining.