Cell Biology Antibodies 11

Anti-PCDHA13 Antibody (CAB7475)

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SKU:
CAB7475
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-PCDHA13 Antibody
Antibody SKU:CAB7475
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 300-360 of human PCDHA13 (NP_061727.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:Mouse brain, Mouse kidney, Rat brain, Rat kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 300-360 of human PCDHA13 (NP_061727.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:GEIR TKGK LDFE EKKL YEIS VEAV DKGN IPMA GHCT LLVE VLDV NDNA PEVT ITSL SLPI R
Gene ID:56136
Uniprot:Q9Y5I0
Cellular Location:Cell membrane, Single-pass type I membrane protein
Calculated MW:87kDa/102kDa
Observed MW:90kDa
Synonyms:PCDHA13, CNR5, CNRN5, CNRS5, CRNR5, PCDH-ALPHA13
Background:This gene is a member of the protocadherin alpha gene cluster, one of three related gene clusters tandemly linked on chromosome five that demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. The alpha gene cluster is composed of 15 cadherin superfamily genes related to the mouse CNR genes and consists of 13 highly similar and 2 more distantly related coding sequences. The tandem array of 15 N-terminal exons, or variable exons, are followed by downstream C-terminal exons, or constant exons, which are shared by all genes in the cluster. The large, uninterrupted N-terminal exons each encode six cadherin ectodomains while the C-terminal exons encode the cytoplasmic domain. These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins that most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been observed and additional variants have been suggested but their full-length nature has yet to be determined.
UniProt Protein Function:PCDHA13: Potential calcium-dependent cell-adhesion protein. May be involved in the establishment and maintenance of specific neuronal connections in the brain. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Membrane protein, integral

Chromosomal Location of Human Ortholog: 5q31

Cellular Component: integral to membrane; plasma membrane

Molecular Function:calcium ion binding

Biological Process: homophilic cell adhesion

NCBI Summary:This gene is a member of the protocadherin alpha gene cluster, one of three related gene clusters tandemly linked on chromosome five that demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. The alpha gene cluster is composed of 15 cadherin superfamily genes related to the mouse CNR genes and consists of 13 highly similar and 2 more distantly related coding sequences. The tandem array of 15 N-terminal exons, or variable exons, are followed by downstream C-terminal exons, or constant exons, which are shared by all genes in the cluster. The large, uninterrupted N-terminal exons each encode six cadherin ectodomains while the C-terminal exons encode the cytoplasmic domain. These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins that most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been observed and additional variants have been suggested but their full-length nature has yet to be determined. [provided by RefSeq, Jul 2008]
UniProt Code:Q9Y5I0
NCBI GenInfo Identifier:9256580
NCBI Gene ID:56136
NCBI Accession:NP_061727.1
UniProt Secondary Accession:Q9Y5I0,O75277,
UniProt Related Accession:Q9Y5I0
Molecular Weight:87,130 Da
NCBI Full Name:protocadherin alpha-13 isoform 1
NCBI Synonym Full Names:protocadherin alpha 13
NCBI Official Symbol:PCDHA13  
NCBI Official Synonym Symbols:CNR5; CNRN5; CNRS5; CRNR5; PCDH-ALPHA13  
NCBI Protein Information:protocadherin alpha-13
UniProt Protein Name:Protocadherin alpha-13
Protein Family:Protocadherin
UniProt Gene Name:PCDHA13  
UniProt Entry Name:PCDAD_HUMAN
Anti-PCDHA13 Antibody (CAB7475)Western blot analysis of extracts of various cell lines, using PCDHA13 antibody (CAB7475) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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