Antibody Name:	Anti-PCDHA12 Antibody
Antibody SKU:	CAB8501
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 713-792 of human PCDHA12 (NP_114070.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	HL-60, NCI-H460, NIH/3T3, Mouse spinal cord, Mouse brain, Mouse lung, Rat spinal cord, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 713-792 of human PCDHA12 (NP_114070.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LTLL LYTA LRCS APPT VSRC APGK PTLV CSSA VGSW SYSQ QRRQ RVCS AESP PKTD LMAF SPSL QLSR EDCL NPPS EVSY
Gene ID:	56137
Uniprot:	Q9UN75
Cellular Location:	Cell membrane, Single-pass type I membrane protein
Calculated MW:	85kDa/101kDa
Observed MW:	102kDa

Synonyms:

PCDHA12, PCDH-ALPHA12

Background:

This gene is a member of the protocadherin alpha gene cluster, one of three related gene clusters tandemly linked on chromosome five that demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. The alpha gene cluster is composed of 15 cadherin superfamily genes related to the mouse CNR genes and consists of 13 highly similar and 2 more distantly related coding sequences. The tandem array of 15 N-terminal exons, or variable exons, are followed by downstream C-terminal exons, or constant exons, which are shared by all genes in the cluster. The large, uninterrupted N-terminal exons each encode six cadherin ectodomains while the C-terminal exons encode the cytoplasmic domain. These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins that most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been observed and additional variants have been suggested but their full-length nature has yet to be determined.

UniProt Protein Function:	PCDHA12: Potential calcium-dependent cell-adhesion protein. May be involved in the establishment and maintenance of specific neuronal connections in the brain. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 5q31
NCBI Summary:	This gene is a member of the protocadherin alpha gene cluster, one of three related gene clusters tandemly linked on chromosome five that demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. The alpha gene cluster is composed of 15 cadherin superfamily genes related to the mouse CNR genes and consists of 13 highly similar and 2 more distantly related coding sequences. The tandem array of 15 N-terminal exons, or variable exons, are followed by downstream C-terminal exons, or constant exons, which are shared by all genes in the cluster. The large, uninterrupted N-terminal exons each encode six cadherin ectodomains while the C-terminal exons encode the cytoplasmic domain. These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins that most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been observed and additional variants have been suggested but their full-length nature has yet to be determined. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9UN75
NCBI GenInfo Identifier:	13878425
NCBI Gene ID:	56137
NCBI Accession:	Q9UN75.1
UniProt Secondary Accession:	Q9UN75,O75278, Q2M1N8,
UniProt Related Accession:	Q9UN75
Molecular Weight:	85,581 Da
NCBI Full Name:	Protocadherin alpha-12
NCBI Synonym Full Names:	protocadherin alpha 12
NCBI Official Symbol:	PCDHA12
NCBI Official Synonym Symbols:	PCDH-ALPHA12
NCBI Protein Information:	protocadherin alpha-12
UniProt Protein Name:	Protocadherin alpha-12
Protein Family:	Protocadherin
UniProt Gene Name:	PCDHA12
UniProt Entry Name:	PCDAC_HUMAN

	Western blot analysis of extracts of various cell lines, using PCDHA12 antibody (CAB8501) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human stomach using PCDHA12 antibody (CAB8501) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human trachea using PCDHA12 antibody (CAB8501) at dilution of 1:100 (40x lens).