Cell Biology Antibodies 1

Anti-PCDH15 Antibody (CAB10086)

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SKU:
CAB10086
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-PCDH15 Antibody
Antibody SKU:CAB10086
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 160-400 of human PCDH15 (NP_001136235.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:100 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:SH-SY5Y, 293T, A-549, Raji, Mouse brain, Mouse liver, Mouse spleen, Rat brain, Rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 160-400 of human PCDH15 (NP_001136235.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:TVNE LTPV GTTI FTGF SGDN GATD IDDG PNGQ IEYV IQYN PDDP TSND TFEI PLML TGNI VLRK RLNY EDKT RYFV IIQA NDRA QNLN ERRT TTTT LTVD VLDG DDLG PMFL PCVL VPNT RDCR PLTY QAAI PELR TPEE LNPI IVTP PIQA IDQD RNIQ PPSD RPGI LYSI LVGT PEDY PRFF HMHP RTAE LSLL EPVN RDFH QKFD LVIK AEQD NGHP LPAF AGLH IEIL DENN QSPY F
Gene ID:65217
Uniprot:Q96QU1
Cellular Location:Cell membrane, Secreted, Single-pass type I membrane protein
Calculated MW:92kDa/106kDa/185kDa/197kDa/216kDa
Observed MW:100kDa
Synonyms:PCDH15, CDHR15, DFNB23, USH1F
Background:This gene is a member of the cadherin superfamily. Family members encode integral membrane proteins that mediate calcium-dependent cell-cell adhesion. It plays an essential role in maintenance of normal retinal and cochlear function. Mutations in this gene result in hearing loss and Usher Syndrome Type IF (USH1F). Extensive alternative splicing resulting in multiple isoforms has been observed in the mouse ortholog. Similar alternatively spliced transcripts are inferred to occur in human, and additional variants are likely to occur.
UniProt Protein Function:Calcium-dependent cell-adhesion protein. Essential for maintenance of normal retinal and cochlear function.
NCBI Summary:This gene is a member of the cadherin superfamily. Family members encode integral membrane proteins that mediate calcium-dependent cell-cell adhesion. It plays an essential role in maintenance of normal retinal and cochlear function. Mutations in this gene result in hearing loss and Usher Syndrome Type IF (USH1F). Extensive alternative splicing resulting in multiple isoforms has been observed in the mouse ortholog. Similar alternatively spliced transcripts are inferred to occur in human, and additional variants are likely to occur. [provided by RefSeq, Dec 2008]
UniProt Code:Q96QU1
NCBI GenInfo Identifier:115387123
NCBI Gene ID:65217
NCBI Accession:NP_149045.3
UniProt Related Accession:Q96QU1
Molecular Weight:
NCBI Full Name:protocadherin-15 isoform CD1-4
NCBI Synonym Full Names:protocadherin related 15
NCBI Official Symbol:PCDH15  
NCBI Official Synonym Symbols:USH1F; CDHR15; DFNB23  
NCBI Protein Information:protocadherin-15
UniProt Protein Name:Protocadherin-15
Protein Family:Protocadherin
UniProt Gene Name:PCDH15  
Anti-PCDH15 Antibody (CAB10086)Western blot analysis of extracts of various cell lines, using PCDH15 antibody (CAB10086) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
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