Anti-PCBD1 Antibody (CAB9426)
- SKU:
- CAB9426
Description
| Antibody Name: | Anti-PCBD1 Antibody (CAB9426) |
| Antibody SKU: | CAB9426 |
| Antibody Size: | 50µL, 100µL |
| Application: | Western blotting, Immunohistochemistry |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthesized peptide derived from human PCBD1. |
| Application: | Western blotting, Immunohistochemistry |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 293T, HepG2, Mouse liver, Mouse kidney, Mouse pancreas, Rat liver, Rat kidney |
| Immunogen: | A synthesized peptide derived from human PCBD1. |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 12kDa |
| Observed MW: | 12KDa |
| Synonyms: | PCBD1, DCOH, PCBD, PCD, PHS, pterin-4-alpha-carbinolamine dehydratase |
| Background: | This gene encodes a member of the pterin-4-alpha-carbinolamine dehydratase family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The encoded protein functions as both a dehydratase involved in tetrahydrobiopterin biosynthesis, and as a cofactor for HNF1A-dependent transcription. A deficiency of this enzyme leads to hyperphenylalaninemia. Alternative splicing results in multiple transcript variants. |
 | Immunohistochemistry of paraffin-embedded rat kidney using PCBD1 Rabbit mAb at dilution of 1:150 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. |
 | Immunohistochemistry of paraffin-embedded mouse kidney using PCBD1 Rabbit mAb at dilution of 1:150 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. |
 | Immunohistochemistry of paraffin-embedded human esophageal cancer using PCBD1 Rabbit mAb at dilution of 1:150 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. |
 | Immunohistochemistry of paraffin-embedded mouse brain using PCBD1 Rabbit mAb at dilution of 1:150 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. |
 | Western blot analysis of extracts of various cell lines, using at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s. |
Additional Information
Product Type: |
Antibody |
Antibody Type: |
Monoclonal Antibody |
Reactivity: |
Human |
Reactivity: |
NHP - Monkey |
Host Species: |
Rabbit |
Isotype: |
IgG |
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