Description
Antibody Name: | Anti-PCBD1 Antibody (CAB9426) |
Antibody SKU: | CAB9426 |
Antibody Size: | 50µL, 100µL |
Application: | Western blotting, Immunohistochemistry |
Reactivity: | Human, Mouse, Rat |
Host Species: | Rabbit |
Immunogen: | A synthesized peptide derived from human PCBD1. |
Application: | Western blotting, Immunohistochemistry |
Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
Reactivity: | Human, Mouse, Rat |
Positive Samples: | 293T, HepG2, Mouse liver, Mouse kidney, Mouse pancreas, Rat liver, Rat kidney |
Immunogen: | A synthesized peptide derived from human PCBD1. |
Purification Method: | Affinity purification |
Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Isotype: | IgG |
Sequence: | Email for sequence |
Cellular Location: | Cytoplasm, Nucleus |
Calculated MW: | 12kDa |
Observed MW: | 12KDa |
Synonyms: | PCBD1, DCOH, PCBD, PCD, PHS, pterin-4-alpha-carbinolamine dehydratase |
Background: | This gene encodes a member of the pterin-4-alpha-carbinolamine dehydratase family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The encoded protein functions as both a dehydratase involved in tetrahydrobiopterin biosynthesis, and as a cofactor for HNF1A-dependent transcription. A deficiency of this enzyme leads to hyperphenylalaninemia. Alternative splicing results in multiple transcript variants. |
Immunohistochemistry of paraffin-embedded rat kidney using PCBD1 Rabbit mAb at dilution of 1:150 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. | |
Immunohistochemistry of paraffin-embedded mouse kidney using PCBD1 Rabbit mAb at dilution of 1:150 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. | |
Immunohistochemistry of paraffin-embedded human esophageal cancer using PCBD1 Rabbit mAb at dilution of 1:150 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. | |
Immunohistochemistry of paraffin-embedded mouse brain using PCBD1 Rabbit mAb at dilution of 1:150 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. | |
Western blot analysis of extracts of various cell lines, using at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s. |