Cell Biology Antibodies 1
Anti-PBEF / NAMPT Antibody (CAB0256)
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- SKU:
- CAB0256
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-PBEF / NAMPT Antibody |
| Antibody SKU: | CAB0256 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human PBEF / NAMPT (NP_005737.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-87MG, HepG2 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human PBEF / NAMPT (NP_005737.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MNPA AEAE FNIL LATD SYKV THYK QYPP NTSK VYSY FECR EKKT ENSK LRKV KYEE TVFY GLQY ILNK YLKG KVVT KEKI QEAK DVYK EHFQ DDVF NEKG WNYI LEKY DGHL PIEI KAVP EGFV IPRG NVLF TVEN TDPE CYWL TNWI ETIL VQSW YPIT VATN SREQ KKIL AKYL LETS GNLD GLEY KLHD FGYR GVSS QETA GIGA SAHL VNFK GTDT VAGL ALIK KYYG TKDP VPGY SVPA AEHS TITA WGKD HEKD AFEH IVTQ FSSV PVSV VSDS |
| Gene ID: | 10135 |
| Uniprot: | P43490 |
| Cellular Location: | Cytoplasm, Nucleus, Secreted |
| Calculated MW: | 55kDa |
| Observed MW: | 56kDa |
| Synonyms: | NAMPT, 1110035O14Rik, PBEF, PBEF1, VF, VISFATIN |
| Background: | This gene encodes a protein that catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein belongs to the nicotinic acid phosphoribosyltransferase (NAPRTase) family and is thought to be involved in many important biological processes, including metabolism, stress response and aging. This gene has a pseudogene on chromosome 10. |
| UniProt Protein Function: | PBEF: an ubiquitous protein originally described as a cytokine which acts on early B-lineage precursor cells. Other reports indicate that PBEF is not a cytokine-like secreted protein but an intracellular protein associated with the cell cycle. Contains a catalytically active nicotinate phosphoribosyltransferase domain, an enzyme involved in nicotinamide adenine dinucleotide (NAD) biosynthesis. |
| UniProt Protein Details: | Protein type:Cell cycle regulation; Transferase; EC 2.4.2.12; Cytokine; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide Chromosomal Location of Human Ortholog: 7q22.3 Cellular Component: extracellular space; cytosol; nucleus Molecular Function:protein binding; protein homodimerization activity; cytokine activity; nicotinate-nucleotide diphosphorylase (carboxylating) activity; drug binding; nicotinamide phosphoribosyltransferase activity Biological Process: circadian rhythm; vitamin metabolic process; positive regulation of smooth muscle cell proliferation; female pregnancy; signal transduction; response to organic cyclic substance; NAD biosynthetic process; cell-cell signaling; NAD metabolic process; nicotinamide metabolic process; positive regulation of cell proliferation; insulin receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; circadian regulation of gene expression; water-soluble vitamin metabolic process; positive regulation of nitric-oxide synthase biosynthetic process |
| NCBI Summary: | This gene encodes a protein that catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein belongs to the nicotinic acid phosphoribosyltransferase (NAPRTase) family and is thought to be involved in many important biological processes, including metabolism, stress response and aging. This gene has a pseudogene on chromosome 10. [provided by RefSeq, Feb 2011] |
| UniProt Code: | P43490 |
| NCBI GenInfo Identifier: | 1172027 |
| NCBI Gene ID: | 10135 |
| NCBI Accession: | P43490.1 |
| UniProt Related Accession: | P43490 |
| Molecular Weight: | |
| NCBI Full Name: | Nicotinamide phosphoribosyltransferase |
| NCBI Synonym Full Names: | nicotinamide phosphoribosyltransferase |
| NCBI Official Symbol: | NAMPT |
| NCBI Official Synonym Symbols: | VF; PBEF; PBEF1; VISFATIN; 1110035O14Rik |
| NCBI Protein Information: | nicotinamide phosphoribosyltransferase |
| UniProt Protein Name: | Nicotinamide phosphoribosyltransferase |
| UniProt Synonym Protein Names: | Pre-B-cell colony-enhancing factor 1; Pre-B cell-enhancing factor; Visfatin |
| UniProt Gene Name: | NAMPT |
| UniProt Entry Name: | NAMPT_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PBEF / NAMPT antibody (CAB0256) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded rat ovary using PBEF / NAMPT antibody (CAB0256) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human colon carcinoma using PBEF / NAMPT antibody (CAB0256) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse spleen using PBEF / NAMPT antibody (CAB0256) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of HeLa cells using PBEF / NAMPT antibody (CAB0256). |
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