Developmental Biology

Anti-PAX5 Antibody (CAB12048)

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SKU:
CAB12048
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Developmental Biology

Description

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Antibody Name:Anti-PAX5 Antibody
Antibody SKU:CAB12048
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PAX5 (NP_057953.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Rat
Positive Samples:K-562, Raji, A-549, Rat heart
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PAX5 (NP_057953.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MDLE KNYP TPRT SRTG HGGV NQLG GVFV NGRP LPDV VRQR IVEL AHQG VRPC DISR QLRV SHGC VSKI LGRY YETG SIKP GVIG GSKP KVAT PKVV EKIA
Gene ID:5079
Uniprot:Q02548
Cellular Location:Nucleus
Calculated MW:31kDa/32kDa/34kDa/35kDa/37kDa/38kDa/42kDa
Observed MW:38kDa
Synonyms:PAX5, ALL3, BSAP
Background:This gene encodes a member of the paired box (PAX) family of transcription factors. The central feature of this gene family is a novel, highly conserved DNA-binding motif, known as the paired box. Paired box transcription factors are important regulators in early development, and alterations in the expression of their genes are thought to contribute to neoplastic transformation. This gene encodes the B-cell lineage specific activator protein that is expressed at early, but not late stages of B-cell differentiation. Its expression has also been detected in developing CNS and testis and so the encoded protein may also play a role in neural development and spermatogenesis. This gene is located at 9p13, which is involved in t(9;14)(p13;q32) translocations recurring in small lymphocytic lymphomas of the plasmacytoid subtype, and in derived large-cell lymphomas. This translocation brings the potent E-mu enhancer of the IgH gene into close proximity of the PAX5 promoter, suggesting that the deregulation of transcription of this gene contributes to the pathogenesis of these lymphomas. Alternative splicing results in multiple transcript variants encoding different isoforms.
UniProt Protein Function:May play an important role in B-cell differentiation as well as neural development and spermatogenesis. Involved in the regulation of the CD19 gene, a B-lymphoid-specific target gene.
NCBI Summary:This gene encodes a member of the paired box (PAX) family of transcription factors. The central feature of this gene family is a novel, highly conserved DNA-binding motif, known as the paired box. Paired box transcription factors are important regulators in early development, and alterations in the expression of their genes are thought to contribute to neoplastic transformation. This gene encodes the B-cell lineage specific activator protein that is expressed at early, but not late stages of B-cell differentiation. Its expression has also been detected in developing CNS and testis and so the encoded protein may also play a role in neural development and spermatogenesis. This gene is located at 9p13, which is involved in t(9;14)(p13;q32) translocations recurring in small lymphocytic lymphomas of the plasmacytoid subtype, and in derived large-cell lymphomas. This translocation brings the potent E-mu enhancer of the IgH gene into close proximity of the PAX5 promoter, suggesting that the deregulation of transcription of this gene contributes to the pathogenesis of these lymphomas. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013]
UniProt Code:Q02548
NCBI GenInfo Identifier:417449
NCBI Gene ID:5079
NCBI Accession:Q02548.1
UniProt Secondary Accession:Q02548,O75933, Q5SFM2, Q6S728, A3QVP6, A3QVP7, A3QVP8 C0KTF6, C0KTF7, C0KTF8, C0KTF9, C0KTG0,
UniProt Related Accession:Q02548
Molecular Weight:34,132 Da
NCBI Full Name:Paired box protein Pax-5
NCBI Synonym Full Names:paired box 5
NCBI Official Symbol:PAX5  
NCBI Official Synonym Symbols:ALL3; BSAP  
NCBI Protein Information:paired box protein Pax-5
UniProt Protein Name:Paired box protein Pax-5
UniProt Synonym Protein Names:B-cell-specific transcription factor; BSAP
Protein Family:Paired box protein
UniProt Gene Name:PAX5  
UniProt Entry Name:PAX5_HUMAN
Anti-PAX5 Antibody (CAB12048)Western blot analysis of extracts of various cell lines, using PAX5 antibody (CAB12048) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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