Cell Biology Antibodies 12

Anti-PARP4 Antibody (CAB8343)

(No reviews yet) Write a Review
SKU:
CAB8343
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-PARP4 Antibody
Antibody SKU:CAB8343
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PARP4 (NP_006428.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:293T, HepG2, Mouse heart, Rat heart
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PARP4 (NP_006428.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MVMG IFAN CIFC LKVK YLPQ QQKK KLQT DIKE NGGK FSFS LNPQ CTHI ILDN ADVL SQYQ LNSI QKNH VHIA NPDF IWKS IREK RLLD VKNY DPYK PLDI TPPP DQKA SSSE VKTE GLCP DSAT EEED TVEL TEFG MQNV EIPH LPQD FEVA KYNT LEKV GMEG GQEA VVVE LQCS RDSR DCPF LISS HFLL DDGM ETRR QFAI KKTS EDAS EYFE NYIE ELKK QGFL LREH FTPE ATQL ASEQ LQAL LLEE VMNS STLS QEVS DLVE MIWA EALG HLEH MLLK PVNR ISLN DVSK AEGI
Gene ID:143
Uniprot:Q9UKK3
Cellular Location:Cytoplasm, Nucleus, cytoskeleton, spindle
Calculated MW:192kDa
Observed MW:193kDa
Synonyms:PARP4, ADPRTL1, ARTD4, PARP-4, PARPL, PH5P, VAULT3, VPARP, VWA5C, p193
Background:This gene encodes poly(ADP-ribosyl)transferase-like 1 protein, which is capable of catalyzing a poly(ADP-ribosyl)ation reaction. This protein has a catalytic domain which is homologous to that of poly (ADP-ribosyl) transferase, but lacks an N-terminal DNA binding domain which activates the C-terminal catalytic domain of poly (ADP-ribosyl) transferase. Since this protein is not capable of binding DNA directly, its transferase activity may be activated by other factors such as protein-protein interaction mediated by the extensive carboxyl terminus.
UniProt Protein Function:PARP4: Component of the vault ribonucleoprotein particle, at least composed of MVP, PARP4 and one or more vault RNAs (vRNAs). Binds to MVP. Associates with TEP1.
UniProt Protein Details:

Protein type:Transferase; EC 2.4.2.30

Chromosomal Location of Human Ortholog: 13q11

Cellular Component: membrane; spindle microtubule; cytoplasm; ribonucleoprotein complex; nucleus

Molecular Function:protein binding; enzyme binding; DNA binding; NAD+ ADP-ribosyltransferase activity

Biological Process: response to drug; cell death; protein amino acid ADP-ribosylation; transport; protein modification process; DNA repair; inflammatory response; response to DNA damage stimulus

NCBI Summary:This gene encodes poly(ADP-ribosyl)transferase-like 1 protein, which is capable of catalyzing a poly(ADP-ribosyl)ation reaction. This protein has a catalytic domain which is homologous to that of poly (ADP-ribosyl) transferase, but lacks an N-terminal DNA binding domain which activates the C-terminal catalytic domain of poly (ADP-ribosyl) transferase. Since this protein is not capable of binding DNA directly, its transferase activity may be activated by other factors such as protein-protein interaction mediated by the extensive carboxyl terminus. [provided by RefSeq, Jul 2008]
UniProt Code:Q9UKK3
NCBI GenInfo Identifier:112789550
NCBI Gene ID:143
NCBI Accession:NP_006428.2
UniProt Secondary Accession:Q9UKK3,O75903, Q14682, Q5QNZ9, Q9H1M6,
UniProt Related Accession:Q9UKK3
Molecular Weight:192,595 Da
NCBI Full Name:poly
NCBI Synonym Full Names:poly (ADP-ribose) polymerase family, member 4
NCBI Official Symbol:PARP4  
NCBI Official Synonym Symbols:PH5P; p193; ARTD4; PARPL; VPARP; VWA5C; PARP-4; VAULT3; ADPRTL1  
NCBI Protein Information:poly [ADP-ribose] polymerase 4; poly [ADP-ribose] polymerase 4; 193 kDa vault protein; ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase)-like 1; ADP-ribosyltransferase diphtheria toxin-like 4; H5 proline-rich; I-alpha-I-related; PARP-related; PARP-related/IalphaI-related H5/proline-rich; poly(ADP-ribose) synthetase; poly(ADP-ribosyl)transferase-like 1; vault poly(ADP-ribose) polymerase; vault protein, 193-kDa; von Willebrand factor A domain containing 5C
UniProt Protein Name:Poly [ADP-ribose] polymerase 4
UniProt Synonym Protein Names:193 kDa vault protein; ADP-ribosyltransferase diphtheria toxin-like 4; ARTD4; PARP-related/IalphaI-related H5/proline-rich; PH5P; Vault poly(ADP-ribose) polymerase; VPARP
Protein Family:Poly [ADP-ribose] polymerase
UniProt Gene Name:PARP4  
UniProt Entry Name:PARP4_HUMAN
Anti-PARP4 Polyclonal Antibody (CAB8343)Western blot analysis of extracts of various cell lines, using PARP4 antibody (CAB8343) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
View AllClose

0 Reviews

View AllClose

Related Products