Epigenetics & Nuclear Signaling Antibodies 1

Anti-PARP10 Antibody (CAB12815)

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SKU:
CAB12815
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-PARP10 Antibody
Antibody SKU:CAB12815
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PARP10 (NP_116178.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:HepG2, 293T, HT-29, MCF7, HeLa
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PARP10 (NP_116178.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MVAM AEAE AGVA VEVR GLPP AVPD ELLT LYFE NRRR SGGG PVLS WQRL GCGG VLTF REPA DAER VLAQ ADHE LHGA QLSL RPAP PRAP ARLL LQGL PPGT TPQR LEQH VQAL LRAS GLPV QPCC ALAS PRPD RALV QLPK PLSE ADVR VLEE QAQN LGLE GTLV SLAR VPQA RAVR VVGD GASV DLLL LELY LENE RRSG GGPL EDLQ RLPG PLGT VASF QQWQ VAER VLQQ EHRL QGSE LSLV PHYD ILEP EELA ENTS GGDH PSTQ GPRA TKHA LLRT GGLV TALQ GAGT VTMG SGEE
Gene ID:84875
Uniprot:Q53GL7
Cellular Location:Cytoplasm, Nucleus, nucleolus
Calculated MW:109kDa
Observed MW:110kDa
Synonyms:PARP10, ARTD10
Background:
UniProt Protein Function:PARP10: May play a role in cell proliferation. May be required for the maintenance of cell cycle progression.
UniProt Protein Details:

Protein type:EC 2.4.2.30; Transferase; Nucleolus

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: cytoplasm; Golgi apparatus; nucleoplasm; nucleus

Molecular Function:NAD+ ADP-ribosyltransferase activity; protein binding

Biological Process: inhibition of NF-kappaB transcription factor; negative regulation of fibroblast proliferation; negative regulation of protein import into nucleus, translocation; negative regulation of viral genome replication; protein amino acid ADP-ribosylation

NCBI Summary:Poly(ADP-ribose) polymerases (PARPs), such as PARP10, regulate gene transcription by altering chromatin organization by adding ADP-ribose to histones. PARPs can also function as transcriptional cofactors (Yu et al., 2005 [PubMed 15674325]).[supplied by OMIM, Mar 2008]
UniProt Code:Q53GL7
NCBI GenInfo Identifier:116248563
NCBI Gene ID:84875
NCBI Accession:Q53GL7.2
UniProt Secondary Accession:Q53GL7,Q8N2I0, Q8WV05, Q96CH7, Q96K72,
UniProt Related Accession:Q53GL7
Molecular Weight:109998 Da
NCBI Full Name:Poly
NCBI Synonym Full Names:poly(ADP-ribose) polymerase family member 10
NCBI Official Symbol:PARP10  
NCBI Official Synonym Symbols:ARTD10  
NCBI Protein Information:poly [ADP-ribose] polymerase 10
UniProt Protein Name:Poly [ADP-ribose] polymerase 10
UniProt Synonym Protein Names:ADP-ribosyltransferase diphtheria toxin-like 10; ARTD10
Protein Family:Poly [ADP-ribose] polymerase
UniProt Gene Name:PARP10  
UniProt Entry Name:PAR10_HUMAN
Anti-PARP10 Antibody (CAB12815)Western blot analysis of extracts of various cell lines, using PARP10 antibody (CAB12815) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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