Antibody Name:	PARL Rabbit Polyclonal Antibody
Antibody SKU:	CAB8232
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human PARL (NP_001032728.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human
Positive Samples:	HepG2, MCF7

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human PARL (NP_001032728.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAWR GWAQ RGWG CGQA WGAS VGGR SCEE LTAV LTPP QLLG RRFN FFIQ QKCG FRKA PRKV EPRR SDPG TSGE AYKR SALI PPVE ETVF YPSP YPIR SLIK PLFF TVGF TGCA FGSA AIWQ YESL KSRV QSYF DGIK ADWL DSIR PQKE GDFR KEIN KWWN NLSD GQRT VT
Gene ID:	55486
Uniprot:	Q9H300
Cellular Location:	Mitochondrion inner membrane, Multi-pass membrane protein, Nucleus
Calculated MW:	36kDa/42kDa
Observed MW:	49kDa

Synonyms:

PARL, PRO2207, PSARL, PSARL1, PSENIP2, RHBDS1

Background:

This gene encodes a member of the rhomboid family of intramembrane serine proteases that is localized to the inner mitochondrial membrane. The encoded protein regulates mitochondrial remodeling and apoptosis through regulated substrate proteolysis. Proteolytic processing of the encoded protein results in the release of a small peptide, P-beta, which may transit to the nucleus. Mutations in this gene may be associated with Parkinson's disease.

UniProt Protein Function:	PSARL: a mitochondrial intramembrane protease that is associated with insulin resistance and type 2 diabetes. Induced in humans by caloric restriction. Required for the control of apoptosis during postnatal growth. Essential for proteolytic processing of an antiapoptotic form of OPA1 which prevents the release of mitochondrial cytochrome c in response to intrinsic apoptotic signals. Promotes changes in mitochondria morphology regulated by phosphorylation of P-beta domain. Induced in humans by caloric restriction. Interacts with PSEN1 and PSEN2. Binds OPA1. Two isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Apoptosis; Membrane protein, integral; Protease; EC 3.4.21.105; Membrane protein, multi-pass; Mitochondrial Chromosomal Location of Human Ortholog: 3q27.1 Cellular Component: mitochondrion; mitochondrial inner membrane; integral to membrane; nucleus Molecular Function:protein binding; serine-type endopeptidase activity; endopeptidase activity Biological Process: membrane protein proteolysis; proteolysis
NCBI Summary:	This gene encodes a mitochondrial integral membrane protein. Following proteolytic processing of this protein, a small peptide (P-beta) is formed and translocated to the nucleus. This gene may be involved in signal transduction via regulated intramembrane proteolysis of membrane-tethered precursor proteins. Variation in this gene has been associated with increased risk for type 2 diabetes. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9H300
NCBI GenInfo Identifier:	143811433
NCBI Gene ID:	55486
NCBI Accession:	Q9H300.2
UniProt Secondary Accession:	Q9H300,Q96CQ4, Q9BTJ6, Q9P1E3,
UniProt Related Accession:	Q9H300
Molecular Weight:	36,589 Da
NCBI Full Name:	Presenilins-associated rhomboid-like protein, mitochondrial
NCBI Synonym Full Names:	presenilin associated, rhomboid-like
NCBI Official Symbol:	PARL
NCBI Official Synonym Symbols:	PSARL; PSARL1; RHBDS1; PRO2207; PSENIP2
NCBI Protein Information:	presenilins-associated rhomboid-like protein, mitochondrial; rhomboid 7 homolog 1; mitochondrial intramembrane cleaving protease PARL; mitochondrial intramembrane-cleaving protease PARL
UniProt Protein Name:	Presenilins-associated rhomboid-like protein, mitochondrial
UniProt Synonym Protein Names:	Mitochondrial intramembrane cleaving protease PARLP-beta; Pbeta
Protein Family:	Presenilins-associated rhomboid-like protein
UniProt Gene Name:	PARL
UniProt Entry Name:	PARL_HUMAN

	Western blot analysis of extracts of various cell lines, using PARL antibody (CAB8232) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of U2OS cells using PARL antibody (CAB8232) at dilution of 1:100. Blue: DAPI for nuclear staining.