Epigenetics & Nuclear Signaling Antibodies 1
Anti-PARG Antibody (CAB10577)
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- SKU:
- CAB10577
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-PARG Antibody |
| Antibody SKU: | CAB10577 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 777-976 of human PARG (NP_003622.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:1000 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | A375, K-562, U-251MG, LO2, HeLa, NIH/3T3, Mouse liver, Mouse brain, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 777-976 of human PARG (NP_003622.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | HNEC LIIT GTEQ YSEY TGYA ETYR WSRS HEDG SERD DWQR RCTE IVAI DALH FRRY LDQF VPEK MRRE LNKA YCGF LRPG VSSE NLSA VATG NWGC GAFG GDAR LKAL IQIL AAAA AERD VVYF TFGD SELM RDIY SMHI FLTE RKLT VGDV YKLL LRYY NEEC RNCS TPGP DIKL YPFI YHAV ESCA ETAD HSGQ RTGT |
| Gene ID: | 8505 |
| Uniprot: | Q86W56 |
| Cellular Location: | Cytoplasm, Mitochondrion, Mitochondrion matrix, Nucleus |
| Calculated MW: | 54kDa/60kDa/99kDa/102kDa/111kDa |
| Observed MW: | 111kDa |
| Synonyms: | PARG, PARG99 |
| Background: | Poly(ADP-ribose) glycohydrolase (PARG) is the major enzyme responsible for the catabolism of poly(ADP-ribose), a reversible covalent-modifier of chromosomal proteins. The protein is found in many tissues and may be subject to proteolysis generating smaller, active products. Several transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | PARG: Poly(ADP-ribose) synthesized after DNA damage is only present transiently and is rapidly degraded by poly(ADP-ribose) glycohydrolase. Poly(ADP-ribose) metabolism may be required for maintenance of the normal function of neuronal cells. Belongs to the poly(ADP-ribose) glycohydrolase family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 3.2.1.143; Hydrolase Chromosomal Location of Human Ortholog: 10q11.23 Cellular Component: cytoplasm; cytosol; intracellular membrane-bound organelle; nucleoplasm; nucleus Molecular Function:poly(ADP-ribose) glycohydrolase activity; protein binding |
| NCBI Summary: | Poly(ADP-ribose) glycohydrolase (PARG) is the major enzyme responsible for the catabolism of poly(ADP-ribose), a reversible covalent-modifier of chromosomal proteins. The protein is found in many tissues and may be subject to proteolysis generating smaller, active products. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2015] |
| UniProt Code: | Q86W56 |
| NCBI GenInfo Identifier: | 56417893 |
| NCBI Gene ID: | 8505 |
| NCBI Accession: | Q86W56.1 |
| UniProt Secondary Accession: | Q86W56,Q6E4P6, Q6E4P7, Q7Z742, Q9Y4W7, A5YBK3, B2RC24 B4DIU5, B4DYR4, I6RUV3, |
| UniProt Related Accession: | Q86W56 |
| Molecular Weight: | Calculated: 54kDa/60kDa/99kDa/102kDa/111kDaObserved: 111kDa |
| NCBI Full Name: | Poly(ADP-ribose) glycohydrolase |
| NCBI Synonym Full Names: | poly(ADP-ribose) glycohydrolase |
| NCBI Official Symbol: | PARG |
| NCBI Official Synonym Symbols: | PARG99 |
| NCBI Protein Information: | poly(ADP-ribose) glycohydrolase |
| UniProt Protein Name: | Poly(ADP-ribose) glycohydrolase |
| Protein Family: | Poly(ADP-ribose) glycohydrolase |
| UniProt Gene Name: | PARG |
 | Western blot analysis of extracts of various cell lines, using PARG antibody (CAB10577) at 1:7000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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