Name: Anti-PARD6A Antibody (CAB12755)
Brand: Cell Cycle Antibodies 1
SKU: CAB12755
Availability: OutOfStock

Antibody Name:	Anti-PARD6A Antibody
Antibody SKU:	CAB12755
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 256-345 of human PARD6A (NP_001032358.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Rat
Positive Samples:	Rat skeletal muscle

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 256-345 of human PARD6A (NP_001032358.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	RGAS GRLT GPPS AGPG PAEP DSDD DSSD LVIE NRQP PSSN GLSQ GPPC WDLH PGCR HPGT RSSL PSLD DQEQ ASSG WGSR IRGD GSGF SL
Gene ID:	50855
Uniprot:	Q9NPB6
Cellular Location:	Cell junction, Cell membrane, Cell projection, Cytoplasm, ruffle, tight junction
Calculated MW:	37kDa
Observed MW:	37kDa

Synonyms:

PARD6A, PAR-6A, PAR6, PAR6C, PAR6alpha, TAX40, TIP-40

Background:

This gene is a member of the PAR6 family and encodes a protein with a PSD95/Discs-large/ZO1 (PDZ) domain and a semi-Cdc42/Rac interactive binding (CRIB) domain. This cell membrane protein is involved in asymmetrical cell division and cell polarization processes as a member of a multi-protein complex. The protein also has a role in the epithelial-to-mesenchymal transition (EMT) that characterizes the invasive phenotype associated with metastatic carcinomas. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

UniProt Protein Function:	PARD6A: Adapter protein involved in asymmetrical cell division and cell polarization processes. Probably involved in the formation of epithelial tight junctions. Association with PARD3 may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins. Interacts with MAP2K5. Interacts with PARD3. Interacts with GTP-bound forms of CDC42, ARHQ/TC10 and RAC1. Interacts with the N-terminal part of PRKCI and PRKCZ. Part of a complex with PARD3, CDC42 or RAC1 and PRKCI or PRKCZ. Part of a complex with LLGL1 and PRKCI. Interacts with human T-cell leukemia virus type I TAX protein. Interacts with MPP5 and CRB3. Interacts with TGFBR1; involved in TGF-beta induced epithelial to mesenchymal transition. Interacts with ECT2 ('Thr- 359' phosphorylated form) and PRKCI. Expressed in pancreas, skeletal muscle, brain and heart. Weakly expressed in kidney and placenta. Belongs to the PAR6 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Adaptor/scaffold; Motility/polarity/chemotaxis; Cell adhesion Chromosomal Location of Human Ortholog: 16q22.1 Cellular Component: ruffle; protein complex; tight junction; apical part of cell; plasma membrane; cell cortex; nucleus; cytosol Molecular Function:protein binding; Rho GTPase binding; protein kinase C binding; GTP-dependent protein binding; transcription factor binding Biological Process: intercellular junction assembly and maintenance; viral reproduction; cell division; transforming growth factor beta receptor signaling pathway; intercellular junction maintenance; cell cycle
NCBI Summary:	This gene is a member of the PAR6 family and encodes a protein with a PSD95/Discs-large/ZO1 (PDZ) domain and a semi-Cdc42/Rac interactive binding (CRIB) domain. This cell membrane protein is involved in asymmetrical cell division and cell polarization processes as a member of a multi-protein complex. The protein also has a role in the epithelial-to-mesenchymal transition (EMT) that characterizes the invasive phenotype associated with metastatic carcinomas. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9NPB6
NCBI GenInfo Identifier:	30913215
NCBI Gene ID:	50855
NCBI Accession:	Q9NPB6.1
UniProt Related Accession:	Q9NPB6
Molecular Weight:	37kDa
NCBI Full Name:	Partitioning defective 6 homolog alpha
NCBI Synonym Full Names:	par-6 family cell polarity regulator alpha
NCBI Official Symbol:	PARD6A
NCBI Official Synonym Symbols:	PAR6; PAR6C; TAX40; PAR-6A; TIP-40; PAR6alpha
NCBI Protein Information:	partitioning defective 6 homolog alpha
UniProt Protein Name:	Partitioning defective 6 homolog alpha
UniProt Synonym Protein Names:	PAR6C; Tax interaction protein 40; TIP-40
UniProt Gene Name:	PARD6A
UniProt Entry Name:	PAR6A_HUMAN

Western blot analysis of extracts of rat skeletal muscle, using PARD6A antibody (CAB12755) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 15s.

Antibodies

Anti-PARD6A Antibody (CAB3064)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)