Cell Biology Antibodies 4

Anti-PAPSS1 Antibody (CAB13773)

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SKU:
CAB13773
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-PAPSS1 Antibody
Antibody SKU:CAB13773
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 190-260 of human PAPSS1 (NP_005434.4).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:LO2, HT-29, 293T, Mouse brain, Rat kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 190-260 of human PAPSS1 (NP_005434.4).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:SEYE KPEA PELV LKTD SCDV NDCV QQVV ELLQ ERDI VPVD ASYE VKEL YVPE NKLH LAKT DAET LPAL KIN
Gene ID:9061
Uniprot:O43252
Cellular Location:
Calculated MW:70kDa
Observed MW:71kDa
Synonyms:PAPSS1, ATPSK1, PAPSS, SK1
Background:
UniProt Protein Function:Bifunctional enzyme with both ATP sulfurylase and APS kinase activity, which mediates two steps in the sulfate activation pathway. The first step is the transfer of a sulfate group to ATP to yield adenosine 5'-phosphosulfate (APS), and the second step is the transfer of a phosphate group from ATP to APS yielding 3'-phosphoadenylylsulfate (PAPS: activated sulfate donor used by sulfotransferase). In mammals, PAPS is the sole source of sulfate; APS appears to be only an intermediate in the sulfate-activation pathway (PubMed:9576487, PubMed:9668121, PubMed:9648242, PubMed:14747722). Required for normal biosynthesis of sulfated L-selectin ligands in endothelial cells (PubMed:9576487).
NCBI Summary:Three-prime-phosphoadenosine 5-prime-phosphosulfate (PAPS) is the sulfate donor cosubstrate for all sulfotransferase (SULT) enzymes (Xu et al., 2000 [PubMed 10679223]). SULTs catalyze the sulfate conjugation of many endogenous and exogenous compounds, including drugs and other xenobiotics. In humans, PAPS is synthesized from adenosine 5-prime triphosphate (ATP) and inorganic sulfate by 2 isoforms, PAPSS1 and PAPSS2 (MIM 603005).[supplied by OMIM, Mar 2008]
UniProt Code:O43252
NCBI GenInfo Identifier:46094058
NCBI Gene ID:9061
NCBI Accession:NP_005434
UniProt Secondary Accession:O43252,O43841, O75332, Q6IAX6, Q96FB1, Q96TF4, Q9P1P9 Q9UE98,
UniProt Related Accession:O43252
Molecular Weight:70.9 kDa (626aa)
NCBI Full Name:bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1
NCBI Synonym Full Names:3'-phosphoadenosine 5'-phosphosulfate synthase 1
NCBI Official Symbol:PAPSS1  
NCBI Official Synonym Symbols:SK1; PAPSS; ATPSK1  
NCBI Protein Information:bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1
UniProt Protein Name:Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1
UniProt Synonym Protein Names:3'-phosphoadenosine-5'-phosphosulfate synthase; APS kinase; Adenosine-5'-phosphosulfate 3'-phosphotransferase; Adenylylsulfate 3'-phosphotransferase
Protein Family:Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase
UniProt Gene Name:PAPSS1  
Anti-PAPSS1 Antibody (CAB13773)Western blot analysis of extracts of various cell lines, using PAPSS1 antibody (CAB13773) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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