Cell Cycle Antibodies 1
Anti-PAPD5 Antibody (CAB15885)
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- SKU:
- CAB15885
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Description
| Antibody Name: | Anti-PAPD5 Antibody |
| Antibody SKU: | CAB15885 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 480-590 of human PAPD5 (NP_001035374.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, Mouse liver, Rat thymus |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 480-590 of human PAPD5 (NP_001035374.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | YYPN NETE SILG RIIR VTDE VATY RDWI SKQW GLKN RPEP SCNG NGVT LIVD TQQL DKCN NNLS EENE ALGK CRSK TSES LSKH SSNS SSGP VSSS SATQ SSSS DVDS DAT |
| Gene ID: | 64282 |
| Uniprot: | Q8NDF8 |
| Cellular Location: | Cytoplasm, Nucleus, nucleolus |
| Calculated MW: | 41kDa/51kDa/63kDa/64kDa/75kDa |
| Observed MW: | 90kDa |
| Synonyms: | PAPD5, TRF4-2 |
| Background: |
| UniProt Protein Function: | PAPD5: Plays a role in replication-dependent histone mRNA degradation. May be involved in the terminal uridylation of mature histone mRNAs before their degradation is initiated. DNA polymerase, probably involved in DNA repair. May play a role in sister chromatid cohesion. Belongs to the DNA polymerase type-B-like family. 4 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 2.7.7.19; Nucleolus; Transferase Chromosomal Location of Human Ortholog: 16q12.1 |
| UniProt Code: | Q8NDF8 |
| NCBI GenInfo Identifier: | 59800139 |
| NCBI Gene ID: | 64282 |
| NCBI Accession: | Q8NDF8.2 |
| UniProt Secondary Accession: | Q8NDF8,Q9NW67, Q9Y6C0, B4DV38, |
| UniProt Related Accession: | Q8NDF8 |
| Molecular Weight: | 75,801 Da |
| NCBI Full Name: | Non-canonical poly(A) RNA polymerase PAPD5 |
| NCBI Synonym Full Names: | poly(A) RNA polymerase D5, non-canonical |
| NCBI Official Symbol: | PAPD5 |
| NCBI Official Synonym Symbols: | TRF4-2 |
| NCBI Protein Information: | non-canonical poly(A) RNA polymerase PAPD5 |
| UniProt Protein Name: | Non-canonical poly(A) RNA polymerase PAPD5 |
| UniProt Synonym Protein Names: | PAP-associated domain-containing protein 5; Terminal uridylyltransferase 3; TUTase 3; Topoisomerase-related function protein 4-2; TRF4-2 |
| Protein Family: | Non-canonical poly(A) RNA polymerase |
| UniProt Gene Name: | PAPD5 |
| UniProt Entry Name: | PAPD5_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PAPD5 antibody (CAB15885) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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