Cell Cycle Antibodies 1

Anti-PAPD5 Antibody (CAB15885)

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SKU:
CAB15885
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle

Description

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Antibody Name:Anti-PAPD5 Antibody
Antibody SKU:CAB15885
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 480-590 of human PAPD5 (NP_001035374.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, Mouse liver, Rat thymus
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 480-590 of human PAPD5 (NP_001035374.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:YYPN NETE SILG RIIR VTDE VATY RDWI SKQW GLKN RPEP SCNG NGVT LIVD TQQL DKCN NNLS EENE ALGK CRSK TSES LSKH SSNS SSGP VSSS SATQ SSSS DVDS DAT
Gene ID:64282
Uniprot:Q8NDF8
Cellular Location:Cytoplasm, Nucleus, nucleolus
Calculated MW:41kDa/51kDa/63kDa/64kDa/75kDa
Observed MW:90kDa
Synonyms:PAPD5, TRF4-2
Background:
UniProt Protein Function:PAPD5: Plays a role in replication-dependent histone mRNA degradation. May be involved in the terminal uridylation of mature histone mRNAs before their degradation is initiated. DNA polymerase, probably involved in DNA repair. May play a role in sister chromatid cohesion. Belongs to the DNA polymerase type-B-like family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 2.7.7.19; Nucleolus; Transferase

Chromosomal Location of Human Ortholog: 16q12.1

UniProt Code:Q8NDF8
NCBI GenInfo Identifier:59800139
NCBI Gene ID:64282
NCBI Accession:Q8NDF8.2
UniProt Secondary Accession:Q8NDF8,Q9NW67, Q9Y6C0, B4DV38,
UniProt Related Accession:Q8NDF8
Molecular Weight:75,801 Da
NCBI Full Name:Non-canonical poly(A) RNA polymerase PAPD5
NCBI Synonym Full Names:poly(A) RNA polymerase D5, non-canonical
NCBI Official Symbol:PAPD5  
NCBI Official Synonym Symbols:TRF4-2  
NCBI Protein Information:non-canonical poly(A) RNA polymerase PAPD5
UniProt Protein Name:Non-canonical poly(A) RNA polymerase PAPD5
UniProt Synonym Protein Names:PAP-associated domain-containing protein 5; Terminal uridylyltransferase 3; TUTase 3; Topoisomerase-related function protein 4-2; TRF4-2
Protein Family:Non-canonical poly(A) RNA polymerase
UniProt Gene Name:PAPD5  
UniProt Entry Name:PAPD5_HUMAN
Anti-PAPD5 Antibody (CAB15885)Western blot analysis of extracts of various cell lines, using PAPD5 antibody (CAB15885) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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