Epigenetics & Nuclear Signaling Antibodies 1
Anti-PAPD4 Antibody (CAB13238)
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- SKU:
- CAB13238
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-PAPD4 Antibody |
| Antibody SKU: | CAB13238 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PAPD4 (NP_776158.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-87MG, BxPC-3, Mouse heart, Rat heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PAPD4 (NP_776158.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MFPN SILG RPPF TPNH QQHN NFFT LSPT VYSH QQLI DAQF NFQN ADLS RAVS LQQL TYGN VSPI QTSA SPLF RGRK RLSD EKNL PLDG KRQR FHSP HQEP TVVN QIVP LSGE RRYS MPPL FHTH YVPD IVRC VPPF REIA FLEP REIT LPEA KDKL SQQI LELF ETCQ QQIS DLKK KELC RTQL QREI QLLF PQSR LFLV GSSL NGFG TRSS DGDL CLVV KEEP CFFQ VNQK TEAR HILT LVHK HFCT RLSG YIER PQLI RAKV PIVK FRDK VSCV EFDL NVNN IVGI RNTF LLRT YAYL |
| Gene ID: | 167153 |
| Uniprot: | Q6PIY7 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 55kDa/56kDa |
| Observed MW: | 54kDa |
| Synonyms: | PAPD4, GLD2, TUT2 |
| Background: |
| UniProt Protein Function: | PAPD4: Cytoplasmic poly(A) RNA polymerase that adds successive AMP monomers to the 3'-end of specific RNAs, forming a poly(A) tail. In contrast to the canonical nuclear poly(A) RNA polymerase, it only adds poly(A) to selected cytoplasmic mRNAs. Does not play a role in replication-dependent histone mRNA degradation. Belongs to the DNA polymerase type-B-like family. GLD2 subfamily. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Transferase; EC 2.7.7.19; RNA processing Chromosomal Location of Human Ortholog: 5q14.1 Molecular Function:polynucleotide adenylyltransferase activity; protein binding Biological Process: RNA polyadenylation |
| UniProt Code: | Q6PIY7 |
| NCBI GenInfo Identifier: | 74737798 |
| NCBI Gene ID: | 167153 |
| NCBI Accession: | Q6PIY7.1 |
| UniProt Secondary Accession: | Q6PIY7,Q86WZ2, Q8N927, |
| UniProt Related Accession: | Q6PIY7 |
| Molecular Weight: | 55,502 Da |
| NCBI Full Name: | Poly(A) RNA polymerase GLD2 |
| NCBI Synonym Full Names: | poly(A) RNA polymerase D4, non-canonical |
| NCBI Official Symbol: | PAPD4 |
| NCBI Official Synonym Symbols: | GLD2; TUT2 |
| NCBI Protein Information: | poly(A) RNA polymerase GLD2 |
| UniProt Protein Name: | Poly(A) RNA polymerase GLD2 |
| UniProt Synonym Protein Names: | PAP-associated domain-containing protein 4; Terminal uridylyltransferase 2; TUTase 2 |
| Protein Family: | Poly(A) RNA polymerase |
| UniProt Gene Name: | PAPD4 |
| UniProt Entry Name: | GLD2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PAPD4 antibody (CAB13238) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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