Cell Biology Antibodies 12

Anti-PAM16 Antibody (CAB9875)

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SKU:
CAB9875
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-PAM16 Antibody
Antibody SKU:CAB9875
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 21-125 of human PAM16 (NP_057153.8).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:Jurkat, Mouse testis
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 21-125 of human PAM16 (NP_057153.8).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:ARAL RQEF AASR AAAD ARGR AGHR SAAA SNLS GLSL QEAQ QILN VSKL SPEE VQKN YEHL FKVN DKSV GGSF YLQS KVVR AKER LDEE LKIQ AQED REKG QMPH T
Gene ID:51025
Uniprot:Q9Y3D7
Cellular Location:Matrix side, Mitochondrion inner membrane, Peripheral membrane protein
Calculated MW:13kDa
Observed MW:14kDa
Synonyms:PAM16, CGI-136, MAGMAS, SMDMDM, TIM16, TIMM16
Background:
UniProt Protein Function:Magmas: Regulates ATP-dependent protein translocation into the mitochondrial matrix. Inhibits DNAJC19 stimulation of HSPA9/Mortalin ATPase activity. Belongs to the TIM16/PAM16 family.
UniProt Protein Details:

Protein type:Mitochondrial

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: mitochondrial matrix; protein complex

Molecular Function:protein binding

Biological Process: cellular protein metabolic process; negative regulation of ATPase activity; ossification; protein import into mitochondrial matrix; protein targeting to mitochondrion

Disease: Spondylometaphyseal Dysplasia, Megarbane-dagher-melki Type

NCBI Summary:This gene encodes a mitochondrial protein involved in granulocyte-macrophage colony-stimulating factor (GM-CSF) signaling. This protein also plays a role in the import of nuclear-encoded mitochondrial proteins into the mitochondrial matrix and may be important in reactive oxygen species (ROS) homeostasis. Mutations in this gene cause Megarbane-Dagher-Melike type spondylometaphyseal dysplasia, an early lethal skeletal dysplasia characterized by short stature, developmental delay and other skeletal abnormalities. [provided by RefSeq, May 2017]
UniProt Code:Q9Y3D7
NCBI GenInfo Identifier:27363461
NCBI Gene ID:51025
NCBI Accession:NP_057153
UniProt Related Accession:Q9Y3D7
Molecular Weight:14kDa
NCBI Full Name:mitochondrial import inner membrane translocase subunit TIM16
NCBI Synonym Full Names:presequence translocase associated motor 16
NCBI Official Symbol:PAM16  
NCBI Official Synonym Symbols:TIM16; MAGMAS; SMDMDM; TIMM16; CGI-136  
NCBI Protein Information:mitochondrial import inner membrane translocase subunit TIM16
UniProt Protein Name:Mitochondrial import inner membrane translocase subunit TIM16
UniProt Synonym Protein Names:Mitochondria-associated granulocyte macrophage CSF-signaling molecule; Presequence translocated-associated motor subunit PAM16
Protein Family:Mitochondrial import inner membrane translocase
UniProt Gene Name:PAM16  
UniProt Entry Name:TIM16_HUMAN
Anti-PAM16 Polyclonal Antibody (CAB9875)Western blot analysis of extracts of various cell lines, using PAM16 antibody (CAB9875) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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