Cell Biology Antibodies 5

Anti-PAM Antibody (CAB15077)

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SKU:
CAB15077
Product Type:
Antibody
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-PAM Antibody
Antibody SKU:CAB15077
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Mouse
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 800 to the C-terminus of human PAM (NP_620176.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Mouse
Positive Samples:
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 800 to the C-terminus of human PAM (NP_620176.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:GRVL GRFR GKGS GGLN LGNF FASR KGYS RKGF DRLS TEGS DQEK EDDG SESE EEYS APLP ALAP SSS
Gene ID:5066
Uniprot:P19021
Cellular Location:Membrane, Secreted, Secreted, Single-pass type I membrane protein
Calculated MW:96kDa/98kDa/100kDa/106kDa/108kDa
Observed MW:Refer to figures
Synonyms:PAM, PAL, PHM
Background:This gene encodes a multifunctional protein. The encoded preproprotein is proteolytically processed to generate the mature enzyme. This enzyme includes two domains with distinct catalytic activities, a peptidylglycine alpha-hydroxylating monooxygenase (PHM) domain and a peptidyl-alpha-hydroxyglycine alpha-amidating lyase (PAL) domain. These catalytic domains work sequentially to catalyze the conversion of neuroendocrine peptides to active alpha-amidated products. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.
UniProt Protein Function:Bifunctional enzyme that catalyzes 2 sequential steps in C-terminal alpha-amidation of peptides. The monooxygenase part produces an unstable peptidyl(2-hydroxyglycine) intermediate that is dismutated to glyoxylate and the corresponding desglycine peptide amide by the lyase part. C-terminal amidation of peptides such as neuropeptides is essential for full biological activity.
NCBI Summary:This gene encodes a multifunctional protein. The encoded preproprotein is proteolytically processed to generate the mature enzyme. This enzyme includes two domains with distinct catalytic activities, a peptidylglycine alpha-hydroxylating monooxygenase (PHM) domain and a peptidyl-alpha-hydroxyglycine alpha-amidating lyase (PAL) domain. These catalytic domains work sequentially to catalyze the conversion of neuroendocrine peptides to active alpha-amidated products. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Jan 2016]
UniProt Code:P19021
NCBI GenInfo Identifier:21070984
NCBI Gene ID:5066
NCBI Accession:NP_000910.2
UniProt Related Accession:P19021
Molecular Weight:36kDa
NCBI Full Name:peptidyl-glycine alpha-amidating monooxygenase isoform a
NCBI Synonym Full Names:peptidylglycine alpha-amidating monooxygenase
NCBI Official Symbol:PAM  
NCBI Official Synonym Symbols:PAL; PHM  
NCBI Protein Information:peptidyl-glycine alpha-amidating monooxygenase
UniProt Protein Name:Peptidyl-glycine alpha-amidating monooxygenase
UniProt Synonym Protein Names:Peptidylglycine alpha-hydroxylating monooxygenase (EC:1.14.17.3); PHMPeptidyl-alpha-hydroxyglycine alpha-amidating lyase (EC:4.3.2.5)Alternative name(s):Peptidylamidoglycolate lyase; PAL
Protein Family:Phenylalanine aminomutase
UniProt Gene Name:PAM  
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