Cell Biology Antibodies 3
Anti-PALD1 Antibody (CAB12893)
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- SKU:
- CAB12893
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-PALD1 Antibody |
| Antibody SKU: | CAB12893 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PALD1 (NP_055246.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse kidney, Mouse lung, Mouse heart, Mouse liver, Rat kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PALD1 (NP_055246.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MGTT ASTA QQTV SAGT PFEG LQGS GTMD SRHS VSIH SFQS TSLH NSKA KSII PNKV APVV ITYN CKEE FQIH DELL KAHY TLGR LSDN TPEH YLVQ GRYF LVRD VTEK MDVL GTVG SCGA PNFR QVQG GLTV FGMG QPSL SGFR RVLQ KLQK DGHR ECVI FCVR EEPV LFLR ADED FVSY TPRD KQNL HENL QGLG PGVR VESL ELAI RKEI HDFA QLSE NTYH VYHN TEDL WGEP HAVA IHGE DDLH VTEE VYKR PLFL QPTY RYHR LPLP EQGS PLEA QLDA FVSV LRET PSLL QLRD |
| Gene ID: | 27143 |
| Uniprot: | Q9ULE6 |
| Cellular Location: | Cytoplasm, cytosol |
| Calculated MW: | 96kDa |
| Observed MW: | 97kDa |
| Synonyms: | PALD1, KIAA1274, PALD, paladin |
| Background: |
| UniProt Protein Function: | PALD: Belongs to the paladin family. |
| UniProt Protein Details: | Protein type:Unknown function Chromosomal Location of Human Ortholog: 10q22.1 Cellular Component: cytosol; nucleus Molecular Function:protein binding; protein tyrosine phosphatase activity |
| UniProt Code: | Q9ULE6 |
| NCBI GenInfo Identifier: | 146325028 |
| NCBI Gene ID: | 27143 |
| NCBI Accession: | Q9ULE6.3 |
| UniProt Secondary Accession: | Q9ULE6,Q5JTK7, Q5JTK8, B2RMS1, B9EGC6, |
| UniProt Related Accession: | Q9ULE6 |
| Molecular Weight: | 96,754 Da |
| NCBI Full Name: | Paladin |
| NCBI Synonym Full Names: | phosphatase domain containing, paladin 1 |
| NCBI Official Symbol: | PALD1 |
| NCBI Official Synonym Symbols: | PALD; KIAA1274 |
| NCBI Protein Information: | paladin |
| UniProt Protein Name: | Paladin |
| Protein Family: | Paladin |
| UniProt Gene Name: | PALD1 |
| UniProt Entry Name: | PALD_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PALD1 antibody (CAB12893) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunofluorescence analysis of L929 cells using PALD1 Polyclonal Antibody (CAB12893) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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