Antibody Name:	Anti-PAK6 Antibody
Antibody SKU:	CAB7821
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human PAK6 (NP_064553.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse brain, Mouse spinal cord, Mouse heart, Rat brain, Rat spinal cord

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human PAK6 (NP_064553.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MFRK KKKK RPEI SAPQ NFQH RVHT SFDP KEGK FVGL PPQW QNIL DTLR RPKP VVDP SRIT RVQL QPMK TVVR GSAM PVDG YISG LLND IQKL SVIS SNTL RGRS PTSR RRAQ SLGL LGDE HWAT DPDM YLQS PQSE RTDP HGLY LSCN GGTP AGHK QMPW PEPQ SPRV LPNG LAAK AQSL GPAE FQGA SQRC LQLG ACLQ SSPP GASP PTGT NRHG MKAA KHGS EEAR PQSC LVGS ATGR PGGE GSPS PKTR ESSL KRRL FRSM FLST AA
Gene ID:	56924
Uniprot:	Q9NQU5
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	69kDa/74kDa
Observed MW:	75kDa

Synonyms:

PAK6, PAK5

Background:

This gene encodes a member of a family of p21-stimulated serine/threonine protein kinases, which contain an amino-terminal Cdc42/Rac interactive binding (CRIB) domain and a carboxyl-terminal kinase domain. These kinases function in a number of cellular processes, including cytoskeleton rearrangement, apoptosis, and the mitogen-activated protein (MAP) kinase signaling pathway. The protein encoded by this gene interacts with androgen receptor (AR) and translocates to the nucleus, where it is involved in transcriptional regulation. Changes in expression of this gene have been linked to prostate cancer. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	PAK6: a protein kinase of the STE20 family. Interacts with androgen receptor (AR), and cotranslocates into the nucleus with AR in response to androgen. Highly expressed in testis and prostate tissues.
UniProt Protein Details:	Protein type:Nuclear receptor co-regulator; Protein kinase, Ser/Thr (non-receptor); Protein kinase, STE; Kinase, protein; EC 2.7.11.1; STE group; STE20 family; PAKB subfamily Chromosomal Location of Human Ortholog: 15q14 Cellular Component: cytoplasm; nucleus Molecular Function:protein binding; ATP binding; receptor signaling protein serine/threonine kinase activity Biological Process: regulation of transcription, DNA-dependent; apoptosis; cytoskeleton organization and biogenesis; mitotic cell cycle; protein amino acid phosphorylation
NCBI Summary:	This gene encodes a member of a family of p21-stimulated serine/threonine protein kinases, which contain an amino-terminal Cdc42/Rac interactive binding (CRIB) domain and a carboxyl-terminal kinase domain. These kinases function in a number of cellular processes, including cytoskeleton rearrangement, apoptosis, and the mitogen-activated protein (MAP) kinase signaling pathway. The protein encoded by this gene interacts with androgen receptor (AR) and translocates to the nucleus, where it is involved in transcriptional regulation. Changes in expression of this gene have been linked to prostate cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013]
UniProt Code:	Q9NQU5
NCBI GenInfo Identifier:	23396789
NCBI Gene ID:	56924
NCBI Accession:	Q9NQU5.1
UniProt Secondary Accession:	Q9NQU5,A8K2G2, B3KYB0, G5E9R2,
UniProt Related Accession:	Q9NQU5
Molecular Weight:	681
NCBI Full Name:	Serine/threonine-protein kinase PAK 6
NCBI Synonym Full Names:	p21 protein (Cdc42/Rac)-activated kinase 6
NCBI Official Symbol:	PAK6
NCBI Official Synonym Symbols:	PAK5
NCBI Protein Information:	serine/threonine-protein kinase PAK 6; p21(CDKN1A)-activated kinase 6
UniProt Protein Name:	Serine/threonine-protein kinase PAK 6
UniProt Synonym Protein Names:	PAK-5; p21-activated kinase 6; PAK-6
Protein Family:	Serine/threonine-protein kinase
UniProt Gene Name:	PAK6
UniProt Entry Name:	PAK6_HUMAN

	Western blot analysis of extracts of various cell lines, using PAK6 antibody (CAB7821) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human brain cancer using PAK6 antibody (CAB7821) at dilution of 1:100 (40x lens).