Cell Death Antibodies 1

Anti-PAK4 Antibody (CAB2782)

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SKU:
CAB2782
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Death

Description

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Antibody Name:Anti-PAK4 Antibody
Antibody SKU:CAB2782
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant protein of human PAK4
Application:WB
Recommended Dilution:WB 1:500 - 1:1000
Reactivity:Human
Positive Samples:293T
Immunogen:Recombinant protein of human PAK4
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:10298
Uniprot:O96013
Cellular Location:Cytoplasm
Calculated MW:47kDa/48kDa/54kDa/64kDa
Observed MW:70KDa
Synonyms:PAK4
Background:PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. They serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK4 interacts specifically with the GTP-bound form of Cdc42Hs and weakly activates the JNK family of MAP kinases. PAK4 is a mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
UniProt Protein Function:PAK4: a protein kinase of the STE20 family. A mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Interacts specifically with the GTP-bound form of Cdc42 and weakly activates the JNK family of MAP kinases. Overexpressed in several cancers. Required for ras-dependent anchorage-independent growth of tumor cell lines. Four alternatively spliced human isoforms have been reported.
UniProt Protein Details:

Protein type:Kinase, protein; EC 2.7.11.1; Protein kinase, STE; Protein kinase, Ser/Thr (non-receptor); STE group; STE20 family; PAKB subfamily

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: focal adhesion; Golgi apparatus

Molecular Function:protein binding; receptor signaling protein serine/threonine kinase activity

Biological Process: apoptosis; cell growth; cell migration; cell motility; cell proliferation; cytoskeleton organization and biogenesis; mitotic cell cycle; signal transduction

NCBI Summary:PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. They serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK4 interacts specifically with the GTP-bound form of Cdc42Hs and weakly activates the JNK family of MAP kinases. PAK4 is a mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:O96013
NCBI GenInfo Identifier:12585288
NCBI Gene ID:10298
NCBI Accession:O96013.1
UniProt Secondary Accession:O96013,Q8N4E1, Q8NCH5, Q8NDE3, Q9BU33, Q9ULS8, B4DGG6
UniProt Related Accession:O96013
Molecular Weight:
NCBI Full Name:Serine/threonine-protein kinase PAK 4
NCBI Synonym Full Names:p21 (RAC1) activated kinase 4
NCBI Official Symbol:PAK4  
NCBI Protein Information:serine/threonine-protein kinase PAK 4
UniProt Protein Name:Serine/threonine-protein kinase PAK 4
UniProt Synonym Protein Names:p21-activated kinase 4; PAK-4
Protein Family:Serine/threonine-protein kinase
UniProt Gene Name:PAK4  
UniProt Entry Name:PAK4_HUMAN
Anti-PAK4 Antibody (CAB2782)Western blot analysis of extracts of 293T cells, using PAK4 antibody (CAB2782) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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