Anti-PAK1/PAK2/PAK3 Antibody (CAB21090)
- SKU:
- CAB21090
- Product Type:
- Antibody
- Antibody Type:
- Monoclonal Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
Description
| Antibody Name: | Anti-PAK1/PAK2/PAK3 Antibody (CAB21090) |
| Antibody SKU: | CAB21090 |
| Antibody Size: | 50µL, 100µL |
| Application: | Western blotting |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 350-450 of human PAK1/PAK2/PAK3 (NP_002567.3). |
| Application: | Western blotting |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | SH-SY5Y, HeLa, MCF7, Mouse testis, Rat testis, C6 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 350-450 of human PAK1/PAK2/PAK3 (NP_002567.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | GSLT DVVT ETCM DEGQ IAAV CREC LQAL EFLH SNQV IHRD IKSD NILL GMDG SVKL TDFG FCAQ ITPE QSKR STMV GTPY WMAP EVVT RKAY GPKV DIWS L |
| Cellular Location: | |
| Calculated MW: | 65kDa |
| Observed MW: | 61KDa, 66-68KDa |
| Synonyms: | PAKalpha |
| Background: | This gene encodes a family member of serine/threonine p21-activating kinases, known as PAK proteins. These proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling, and they serve as targets for the small GTP binding proteins Cdc42 and Rac. This specific family member regulates cell motility and morphology. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Apr 2010 |
 | Western blot analysis of extracts of various cell lines, using PAK1/PAK2/PAK3 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s. |
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